紫蘇籽油可改善切除卵巢之大鼠所造成的骨質疏鬆症

缺乏雌激素會增加體內的氧化壓力並導致骨質疏鬆。在多種植物油中，紫蘇 (Perilla frutescens, PO) 籽油的ω-3含量為最高約54~64% ，並且會代謝成EPA和DHA。亦有文獻證實EPA和DHA有很好的抗氧化活性，文獻證實在氧化壓力的環境會抑制成骨細胞的分化和增生，本實驗觀察紫蘇籽油是否可以改善骨質疏鬆症。將SD大鼠分為六組，其中一組做偽手術當作控制組，將二十隻老鼠做卵巢切除手術，並分為OVX與每天餵食紫蘇籽油(0.1%、0.5%、1%)，八週後取血液生化值與股骨觀察其骨小樑結構與蛋白表現量。研究結果發現卵巢切除大鼠的大腿骨內脂肪和成骨細胞增加而骨小樑密度降低，在餵食紫蘇籽油後明顯改善。OVX大鼠血中鹼性磷酸酶 (ALP) 濃度明顯增加，給予PO後可有效降低ALP 值。結果顯示卵巢切除之大鼠餵食PO後氧化型低密度脂蛋白比起正常組別也有明顯減少。在我們的研究發現OVX之母鼠Runx2明顯降低，餵食PO後有回復情形。缺乏卵巢之母鼠的RANK與RANKL增加，而餵食PO後降低其表現量。此外，我們在細胞模式中探討PO是否可保護MC3T3-E1免於氧化型LDL誘導氧化壓力所造成的傷害。將MC3T3-E1 細胞培養於含有10μg LDL 和10Μm (CuSO4) 的骨誘導培養液中，並添加不同濃度的PO，進而檢測成骨細胞存活及氧化損傷的指標。氧化型LDL降低ALP 活性明顯低於單純誘導分化組。PO可增加 ALP 的表現及活性。我們的研究指出紫蘇籽油可預防停經症候群所造成的骨質疏鬆症。

關鍵字

骨質疏鬆；停經症候群；紫蘇籽油；氧化壓力

並列摘要

Estrogen deficiency can increase oxidative stress and cause osteoporosis. In comparing to other plant oils, perilla seed oil (PO) consistently contains higher proportion of ω-3 (ALA) fatty acids at 54–64%, and it metabolized into eicosapentaenoic acid (EPA; 20:5) and docosahexaenoic acid (DHA; 22:6). The previous studies showed EPA/DHA possessed a good antioxidant activity. This study aims to explore whether PO can improve osteoporosis. Furthermore, it is determined whether PO could protect osteoblasts from oxLDL induced oxidative stress in the MC3T3-E1 cells. Female Sprague-Dawley rats were divided into 6 experimental groups, five days after ovariectomy, 20 ovariectomized (OVX) rats were divided into 4 groups, randomized to treat with PO (0.1%, 0.5%, 1%) oral gauge daily for 8 weeks, sham were used as controls. At the end of treatment serum biochemical parameters measure. Femurs were removed and tested for trabecular micro-architecture and protein expression. MC3T3-E1 cells were cultured in the osteogenic medium with or without 10μg/mL and 10mM (CuSO4) or different concentrations of PO. The results show the ratio of adipocyte/osteoblast increase and trabecular bone density decrease dominantly in femur bone of ovariectomic rat, and perilla seed oil ameliorates the status significantly. Previous studies demonstrate oxidative protein products inhibit the proliferation and differentiation of rat osteoblasts via oxidative stress. In this sduty, the expression of oxLDL decreased after feed PO in ovariectomic rat. The expression of Heme oxygenase-1 (HO-1) shows the oxidative status in individual. Herein, HO-1 is decreased in bone tissue of ovariectomic rat, but it is restored after treating with PO. RANK and RANKL are important components to regulate osteoclast proliferation. The expression of RANK/RANKL increased in femur bone of ovariectomic rat, and perilla seed oil can reduce the expression of RANK/RANKL. MC3T3-E1 cells were cultured in the osteogenic medium with or without 10μg and 10Μm (CuSO4) or different concentrations of PO, and markers of osteoblast function and oxidative stress were examined. As compared with differentiation incubation, ALP activity decreased after treating with oxLDL, and PO can increase the expression of ALP activity in osteoblastic MC3T3-E1 cells under the presence of oxLDL. Taken together, the results show that perilla seed oil has the potential to prevent women from osteoporosis in postmenopausal syndrome.

並列關鍵字

Osteoporosis ； postmenopausal syndrome ； perilla seed oil ； oxidative stress

參考文獻

1. Shuler, F.D., J. Conjeski, D. Kendall, and J. Salava, Understanding the burden of osteoporosis and use of the World Health Organization FRAX. Orthopedics, 2012. 35(9): p. 798-805.

2. Das, S. and J.C. Crockett, Osteoporosis - a current view of pharmacological prevention and treatment. Drug Des Devel Ther, 2013. 7: p. 435-48.

3. Shao, C.J., Y.H. Hsieh, C.H. Tsai, and K.A. Lai, A nationwide seven-year trend of hip fractures in the elderly population of Taiwan. Bone, 2009. 44(1): p. 125-9.

5. Berger, M.G. and J. Chassagne, [Bone marrow mesenchymal stem cells: from characterization to therapeutic use in adults and children]. Bull Cancer, 2003. 90(8-9): p. 771-8.

6. Bernstein, C.N. and W.D. Leslie, The pathophysiology of bone disease in gastrointestinal disease. Eur J Gastroenterol Hepatol, 2003. 15(8): p. 857-64.

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紫蘇籽油可改善切除卵巢之大鼠所造成的骨質疏鬆症

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