網版印刷碳電極表面修飾生物探針之初步探討

Preliminary study of labeling biological probes on screen- printed carbon electrode

蔡香素(Hsiang-Su Tsai)

指導教授 ： 蔡惠燕

中山醫學大學/健康管理學院/應用化學系碩士班/碩士(2013年)

https://doi.org/10.6834/CSMU.2013.00225

摘要

生物指標成分(Biomarker)的檢測，在臨床上是疾病早期診斷的重要方法，在生化領域中常使用酵素或螢光免疫分析方法(Immunoassay)，其操作繁複、耗時，且儀器設備昂貴，電化學儀器則相對便宜，設備可微小化攜帶方便且可即時偵測，因此發展電化學生物感測方法用來作生物指標成分的檢測，有潛力發展為對疾病做最即時的偵測達到point-of-care的功用，且電化學的敏感度高，偵測極限低，對biomarker的偵測可達到早期發現早期治療。而在製備生物感測器之前，首先要對電極表面做預處理，修飾上生物感測探針(biomarker probes)。本篇論文研究方向是以發展網版印刷碳電極結合電化學生物感測系統為主，初步探討網版印刷碳電極作為生物感測探針的修飾方法。
本研究採用低成本的網版印刷碳電極，初步探討電化學法活化電極、標示蛋白質及幾丁聚醣(Chitosan)之效果，亦探討不同交連劑使生物分子辨識原件接在幾丁聚醣上之效果。每一修飾步驟皆藉由循環伏安法來驗證電極修飾的結果，亦利用電化學阻抗分析方法量測電極表面的變化。結果顯示電極經由電化學預處理後，藉由Glutaraldehyde交聯Chitosan和牛血清蛋白(BSA)是能修飾於網版印刷碳電極上，且可得到再現性較佳之感測元件。

關鍵字

生物感測探針 ； 網版印刷碳電極 ； 幾丁聚醣

並列摘要

The detection of biomarker is important for early disease diagnosis. Enzymes and fluorescence immunoassay are often used, however the procedures are complicated and time-consuming.The costs of the instruments of enzymes and fluorescence immunoassay are expensive. An alternative method, the electrochemistry biosensor system was developed, which instruments are tiny, portable and able to process immediate detection, as a result, it is potentially achieve point-of-care. Electrochemical biosensing system is highly sensitive it’s detection limit is low. The first step of preparation of biosensors, is to label biomarker probes on the electrode. This research is aimed to the development of screen-printed carbon electrode coupled with electrochemical biosensor systems.
In this research, we use low-cost screen-printed carbon electrode, to study the effect of electrochemical to active the electrode, labeled with protein modified with chitosan and explore the effect of different cross-linking agent to identify biological molecules connect with chitosan. Cyclic voltammetry and electrochemical impedance measurement were used to verify the change of electrode surface for early modification. The results showed that the electrochemical method to active the electrode active, then use glutaralde and chitosan mix solution to form a bonding with bovine serum albumin (BSA) on the screen-printed carbon electrode. It can be achieved better reproducibility of sensing components.

並列關鍵字

Biomarker probes ； Screen-printed-crbon electrode ； Chitosan

參考文獻

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