- 學位論文
多流道流體腔之設計及製造與細胞培養觀察
Design / manufacturing of Multi-channel Flow Chamber and Cell Culture
摘要
現今社會中，研究血管細胞於人體血液循環中的的生理變化對血管病因的探討非常重要，而細胞培養為初步實驗觀察中不可或缺的一部份。本研究主旨為製作一體外循環式細胞培養系統，用來取代舊有的細胞培養方式，並探討以此系統培養之內皮細胞與舊有方式的差別。整體系統架構分為三大部分：多流道流體腔設計、動力設備與調控裝置三部分。而本研究著重於多流道流體腔製作及體外循環式細胞培養。 1.平行板式流體腔之製作與改良：依照參考文獻將腔體依序為擴散段、收縮段與流道，由此三部分所組成。利用繪圖軟體繪製出我們所設計的多流道流體腔外型，再加以製作並加以改良其細胞培養時的便利性。製作出腔體後使用水實際在腔道中流動，檢查腔體有無滲漏情況。 2.體外循環式細胞培養：將內皮細胞置於腔道內，並施以一剪應力於內皮細胞上，觀察其生長變化，並比較與靜態細胞培養的型態差別，進而達到研究流體剪應力對內皮細胞的初步影響。
並列摘要
The effect of a hyperdynamic circulatory state on lining cells of vascular wall is a critical issue in vascular biology. In vitro observation is a feasible method utilized in this regard. The aims of this present project is to design and manufacture a multi-channel flow chamber and evaluate the initial effect of shear stress derived from flow chamber on human vascular endothelial cells (HAECs). 1. An improved design of the multi-channel flow chamber : The multi-channel flow chamber system with three channels connected in parallel (Parallel-plate mode) is designed by means of a graphics software. The flow chamber is divided into three parts: main part of flow chamber, power equipment and control device. 2. Cell culture system : HAECs are seeded on coverslips which are fixed at the bottoms of the channels. The effect of the multi-channel flow chamber system-derived shear stress on morphological alternation of HAECs is investigated.