七次方流場之設計、製作與細胞培養

Design, Manufacture and Cell Culture of 7th Order Stream Line and Shear Stress Induced Channel

莊伯懿(Bo-Yi Zhuang)

指導教授 ： 李文興

國立臺北科技大學/機電學院/製造科技研究所/碩士(2010年)

https://doi.org/10.6841/NTUT.2010.00453

摘要

本研究利用數值分析軟體，成功設計與製造七階曲線流體腔，其提供穩定符合人體之剪應力範圍流體，配合細胞培養技術，可模擬體內且為內皮細胞所處環境。
流體腔設計條件於擴散段之擴散角為5度，面積比為4；收縮段於七階曲線中，收縮比值為4，長寬比值為5；細胞培養段寬度20mm、高度1mm及長度為70mm；將擴散段、收縮段與細胞培養段組合即為流體腔最佳化設計。
本設計除了符合對於細胞培養的各種條件例如：溫度控制於37℃、酸鹼值維持在7.2。還須便利細胞觀察，實驗結果証明內皮細胞可因不同剪應力產生變異，在內皮細胞受到剪應力影響下，細胞將隨著流體流動方向逐漸拉長。而剪應力的增加將造成細胞生長形態變化越是顯著。因此本研究可提供一定的範圍的剪應力且穩定的細胞培養之環境做未來研究之用。

關鍵字

數值分析 ； 流體腔 ； 剪應力 ； 細胞培養

並列摘要

In this study, we successfully used the numerical analysis software to design and manufacture a seven-order curve flow chamber, which provide a stable range of the body shear stress with the technology of cell culture we can simulate the environment for the endothelial cell cultures.
The best flow chamber design data are diffusion angle(θ) 5,and 5:1 area ration(AR) for the diffuser, and contraction ration(CR)4, Aspect Ratio 5 for the contractor, and 20mm width, 1mm height, and 70mm length for cell chamber, respectively. The diffuser, contractor and cell culture flow chamber section shall be the best combination of design.
The design in addition to satisfying the various conditions for cell culture such as temperature control at 37℃ and pH maintained at 7.2 is also facilitated to the observation of cell cultures. Experimental results show that endothelial cells would be grown varied with the different shear stress. With the increasing of shear stress, the endothelial cell will be elongated in the flow direction of in culation fluid. Therefore, this study can provide a certain range of shear stress and a stable environment of cell culture as a future research.

並列關鍵字

numerical analysis ； flow chamber ； shear stress ； cell culture

參考文獻

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