- 學位論文
高壓靜電場對超高分子量聚乙烯顆粒引發老鼠巨噬細胞反應之效應
Effects of electrostatic field on ultra-high molecular weight polyethylene particles induce the reaction of mouse macrophage
摘要
本篇論文探討老鼠巨噬細胞株和不同劑量超高分子量聚乙烯磨耗顆粒，在利用倒置培養系統並施以交流靜電場，給予高電壓低電流的電刺激後產生的反應。以往曾有許多研究探討細胞在各種不同外界刺激下的反應，這些外加的刺激多半為直流靜電場，電磁場，直接通電（直流電或交流電），或是利用機械裝置所產生的應力，鮮少有研究觸及交流靜電場。本實驗利用J774A.1 細胞株作為研究對象，並將其置於0.5、1、1.3、和1.6 kV 的交流高壓靜電場中，利用細胞活性測試和反轉錄聚合酵素鏈鎖反應以及同步定量聚合酵素鏈鎖反應，來評估交流靜電場對於老鼠巨噬細胞生長之影響。研究的結果顯示在此條件之下，在0.5和1 kV靜電場中會活化老鼠巨噬細胞生長活性，亦會促進細胞激素分泌，使TNF-alpha、IL-6、和IL-1beta等免疫效果增強；但是，在1.3和1.6 kV中達到抑制細胞生長和細胞激素分泌的效果。
並列摘要
The aim of this study was to determine the effects of an AC electrostatic field generated from a device with high voltage and low current output on the inverted J774A.1 cell line culture system with different ultra-high molecular weight polyethylene (UHMWPE) particle dosages. Many studies about how cells react and respond to external stimulations of various kinds and types were done before. Most of the external stimulations were DC electrostatic fields, electromagnetic fields, direct electrocution (DC or AC), or even mechanical loadings in these studies, but studies using AC electrostatic field as an external stimulation source were rarely found. This study utilized J774A.1 cell line and UHMWPE particles as the target by putting them under the influence of an AC electrostatic field, which was set to use output parameters of 0.5, 1,1.3,and 1.6 kV . The effects of electrostatic field on J774A.1 cell line were assessed by utilizing various kinds of tests, including cell viability test, reverse transcriptase PCR and real time quantitative PCR. The results showed that under 0.5 and 1 kV, the AC electrostatic field had a up-regulation effect on the cell proliferation and expression level of TNF-alpha, IL-6, and IL-1beta.And under 1.3 and 1.6 kV, the AC electrostatic field had a down-regulation effect on the cell proliferation and expression level of TNF-alpha, IL-6, and IL-1beta.