利用微影製程表面加工技術製造超高分子量聚乙烯與聚醚醚酮之高分子子磨耗顆粒以進行動物實驗探討其對骨溶解之效應

Applying Microfabricated Surface Texture to Generate Ultra-High Molecular Weight Polyethylene (UHMWPE) and Poly-Ether-Ether-Ketone (PEEK) Wear Particles in Vivo to investigate their effects on Osteolysis

廖彥鈞(Yan-Jyun Laio)

指導教授 ： 方旭偉 ； 黃俊雄

國立臺北科技大學/工程學院/化學工程研究所/碩士(2011年)

https://doi.org/10.6841/NTUT.2011.00120

摘要

人工關節摩置換後會因金屬與高分子材料擦後產生磨耗顆粒，會在人體內產生一連串生物反應，例如刺激巨噬細胞（macrophage）分泌細胞激素（cytokine），促進蝕骨細胞（osteoclast）的分化，進而引發骨溶解(osteolysis)現象，造成人工關節發生鬆脫 (aseptic loosening)，必須實施人工關節二次置換。但很難從人體中取得磨耗顆粒。本研究中我們利用加工表面磨耗製造大量之不同高分子材料磨耗顆粒並利用已之建立骨溶解現象動物模型，做更進一步探討，以提供將來作為治療與預防的平台。
故本研究為量產不同材料之高分子磨耗顆粒，利用半導體中的微影製程（microfabrication），在4吋晶圓上進行微米加工表面（surface texture），而加工表面上設計有不同微米尺寸的切割裝置（cutting device），藉此裝置可以置備不同高分子棒材之次微米尺寸磨耗顆粒。進行動物實驗，模擬臨床上置換人工關節之情形，探討材料對骨溶解現象之影響。結果成功以相似尺寸之磨耗顆粒誘發骨溶解之前驅發炎反應，並以Masson染色定量，高交聯聚乙烯在手術後初期的發炎程度也較其他材料小。在實驗六周後的結果發現不同材料之間無明顯差異。推測若能減低磨耗顆粒釋放可減低骨溶解發生之機率。推測高交聯聚乙烯具抗磨耗性，可降低磨耗顆粒釋放進而降低骨溶解現象發生。

關鍵字

超高分子量聚乙烯 ； 高交聯聚乙烯 ； 加工表面 ； 骨溶解

並列摘要

It has been known that wear-debris osetolysis is the main reason for long-term failure in total joint arthroplasty. The debris which was produced from orthopaedic implant induces inflammation and stimulates macrophage to secrete varieties of inflammatory cytokines. These cytokines activate osteoclast differentiation and further cause osteolysis, which is the main cause of aseptic loosening. Thus, there is more wear resistance materials occurred. However, the wear-resistance debris biological response has not been study thoroughly. Nevertheless, it is difficult to obtain sufficient particles from human tissue. Therefore, we used surface texture technology to generate particles. In order to generate quantities of particles from different materials such as UHMWPE, high cross-linked polyethylene (XPE) and poly-ether-ether-ketone (PEEK), we did microfabrication process. We applied microfabricated surface texture on 4-inch silicon wafer and then designed different sizes cutting devices on it. In vivo, we simulated total joint arthroplasty to insert titanium pin into rats’ distal femur and then injected particles we made.
In this study, we successfully generated the similar sizes of particles from different materials and we could observe the wear-induced inflammation. Under almost the same concentration, morphology and size of particles, we found out XPE caused less inflammation than other materials at the beginning of surgery. After 6 weeks of surgery, we found out there was no significant difference of inflammation between different materials. Additionally, it spent more time to abrade XPE particles. We speculated XPE decreases debris generation and causes less inflammation than other materials we used.

並列關鍵字

UHMWPE ； XPE ； surface texture ； oseolysis

參考文獻

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