生醫金屬材料表面改質 - In Vitro 研究

In Vitro Study of Surface Treatment on Titanium and 316L Stainless Steel

王馨偵(Hsin-Chen Wang)

指導教授 ： 薛文景

國立臺北科技大學/工程學院/材料科學與工程研究所/碩士(2007年)

摘要

鈦金屬與316L不鏽鋼，已廣泛用於骨科及牙科植入材料上，相對於鈦金屬，316L不鏽鋼生醫相容性較具爭議，肇因於不鏽鋼表面於植體內的離子釋出而影響細胞的初始增殖與貼覆行為進而降低其生物相容性。本研究將G2醫療用鈦金屬及316L不鏽鋼試片改質，使其表面具有含骨質元素鈣、磷、氧、鈦元素之粗糙活性複合層，增加骨母細胞接觸面積及骨整合性。表面處理後之試片以MG-63 類骨母細胞做In-Vitro觀察利用ELISA reader比較類骨母細胞在各種試片表面增生情況。實驗中，同時提出鈦金屬與316L不鏽鋼表面處理後之各項交流阻抗值分析情況，並與鈦金屬與316L不鏽鋼原材比較，其改質後植體皆可提高粗糙度、抗蝕性並有助於骨母細胞的貼附，浸漬實驗可誘導植體表面類氫氧基磷灰石成核，冀望此研究成果能作為生醫金屬植入金屬在臨床醫學上的進一步參考。

關鍵字

醫療用G2鈦金屬 ； 316L不鏽鋼 ； 生物相容性 ； 活性複合層 ； MG-63類骨母細胞 ； ELISA reader

並列摘要

The most common metals used in orthopedics and dentistry are titanium metal or alloys and 316L stainless steel. The stainless Steel provides higher strength and rather economical cost for the implantation processes. However, usage of 316L stainless steels may be exposed to a process of corrosion in vivo that make them cytotoxic for implant tissues. The aim of this work is to propose a novel treatment of surface roughness with Ca / P / O / Ti active compound layer on G-2 commercial titanium metal and AISI 316L SS, in which the in-vitro beneficiation are effected. The evaluation of biocompatibility for specimens with or without surface treatments is into Hank′s solution for a 14 days period. The compound layer enhanced corrosion resistance and matured bone-like apatite. The MTT results of osteoblasts cells on the metallic substrates indicate that the active layer treated on stainless steel obtains a better cell proliferation and biocompatibility than that of untreated stainless steel.

並列關鍵字

G-2 titanium metal ； 316 L stainless steel ； Hank′s compound layer ； MTT ； biocompatibility

參考文獻

[3] Milella, E., Cosentino, F., Licciulli, A., Massaro, C, “Preparation and characterization of titania/hydroxyapatite composite coatings obtained by sol-gel process, ”Biomaterials 22, 2001, pp. 1425-1431

[4] Oshida, Y., Sellers, C.B., Mirza, K., Farzin-Nia, F.," Corrosion of dental metallic materials by dental treatment agents," Materials Science and Engineering C 25 (3), 2005, pp. 343-348

[5] Morais S, Sousa JP, Fernandes MH, Carvalho GS. “In vitro osteoblastic differentiation of human bone marrow cells in the presence of metal ions .” Journal of Biomedical Materials Research, 44, 1999, pp. 176-190

[7] 蘇建榮，針對不同表面處理之鈦金屬骨螺絲作生物親合性的評估，碩士， 成功大學，台南，90

[9] S.E. Lynch, R.J. Genco, and R.E. Marx,” Tissue engineering: applications in maxillofacial surgery and periodontics,“ Proceedings of SPIE - The International