以靜電紡絲法製備聚乙烯醇/龍膽奈米纖維薄膜於抗菌性及生物相容性之研究

A Stady on the Anti-bacteria and Biocompatibility of Polyvinyl Alcohol/Gentiana Nano-fiber Membranes via Electrospinning Method

許芷萍(Chih-Ping HSU)

指導教授 ： 鄧道興

國立臺北科技大學/工程學院/有機高分子研究所/碩士(2010年)

摘要

聚乙烯醇(PVA)為一生物可分解性材料，具有生物相容性、高度的親水性、良好的成膜性及無毒性，使聚乙烯醇成為近年來具有發產潛力的生醫高分子材料。聚乙烯醇可溶於去離子水中，形成高分子溶液，利用靜電紡絲技術製備具有奈米纖維結構之薄膜。本實驗所使用龍膽萃取液中含有效成分---龍膽苦苷(Gentiopicroside)為龍膽主要生物活性成分具有保肝利膽、抗菌、抗氧化、美白之功效。近年來多被開發於醫療美容之應用。
本研究第一階段利用各種不同操作條件包括PVA溶液濃度、收集器種類不同，觀察其纖維型態，尋找最佳操作參數，結果得知高分子溶液10 wt％，操作電壓20 kV，紡絲距離20cm，滾筒收集方式為最佳條件，所製得纖維薄膜之纖維平均直徑約為327nm。第二階段實驗在PVA高分子溶液中添加不同比例龍膽萃取液，利用最佳條件製備出不同龍膽含量之PVA/Gentiana 纖維複合薄膜，利用SEM觀察纖維平均細度約在216~334nm。以ATR-FTIR對PVA/Gentiana奈米纖維膜做表面特徵官能基分析，發現添加龍膽萃取液並未出現新的官能基特性峰或是造成偏移現象，代表本研究屬於物理混掺。並且對PVA/Gentiana奈米纖維膜做DMA機械性質分析，發現隨著龍膽萃取液添加量的提高機械性質會有下降的趨勢，使用DSC與TGA對熱性質結果做分析。在生物活性方面探討，抗菌實驗中以金黃色葡萄球菌作測試結果發現添加30wt％龍膽萃取液，能達到>99.44%滅菌效果，生物相容性測試中，以L929細胞貼附實驗來探討對PVA/Gentiana奈米纖維膜之表面貼附性，發現添加20 wt%龍膽萃取液其細胞存活率即可達到100%。

關鍵字

聚乙烯醇 ； 龍膽萃取液 ； 靜電紡絲 ； 滾筒收集 ； 抗菌 ； 生物相容性

並列摘要

Polyvinyl Alcohol (PVA) is a biodegradable material that has attracted enormous attention in pharmaceutical and medical industry due to its biocompatibility biodegradability, hight hydrophilicity, non-toxic to human body and good film formability. In this study PVA fibers were electrospun from polymer solution witch PVA powders were dissolved in water at 900C for 2h. The Gentian extract were obtained form marlcet. Its active component gentiopicroside has antioxidant, tyrosinase inhibitory and antibacterial effect using human body.
The first step of this experiment was to find out the best condition to produce PVA polymer fibrous membrane. As polymer fibrous membrane is affected by operating parameters while electrospinning. The best production conditions of PVA fibrous products were studied. It showed that the PVA fibrous with average diameter 240-280nm could be produced under the conditions of 10wt％ polymer solution concentration, 20kV operating voltage and 20cm capillary-screen distance. Meanwhile， the best results were achieved by rolling collection.
The second step of this experiment was to add Gentiana Extract into PVA polymer solution to produce PVA/Gentiana composite nanofibrous membrane. The SEM was applied to investigate the morphology of fibrous membranes and the results showed that its average diameters were in the range of 216~334nm. From the data of ATR-FTIR test, it was concluded that neither new groups were made nor phenomenon of displacement was happened. The mechanical properties of PVA/Gentiana nanofibrous membrane were investigated by DMA. The experimental results showed that the mechanical properties declined with the increasing of Gentiana Extract concentration. The thermal properties were analyzed by DSC and TGA.
The characteristic of inhibition of bacteria growth of PVA fibrous membrane would be achieved 99.44% up adding 30wt% Gentiana Extract. The L929 fibroblast cell attachment to PVA/Gentiana nanofiber membrance were investigated in vitro and 100% Cell viability by adding 20wt% Gentiana Extract.

並列關鍵字

PVA ； Gentiana ； Electrospinning ； Wire drum collector ； Antibiotic ； Biocompatibility

參考文獻

6. Leob S.，Sourirajan S.，"Sea water demineralization by means of an osmotic membrane"，Adv Chem Ser，38，117，1963.

7. C.-K. Yeom，K.-H. Lee* "Pervaporation separation of water-acetic acid mixtures through poly(vinyl alcohol) membranes crosslinked with glutaraldehyde" ，Journal of membrane Science,1996,，109，257-265.

17. Reneker, D.H.,I. Chun, “Nanometre Diameter Fibres of Polymer, Produced by Electrospinning ,” Nanotechnology, Vol. 7, No. 3, 1996, pp.216-223.

18. Kim, J.-S., Reneker, D.H., “Polybenzimidazole Nanofiber Produced by Electrospinning,” Polymer Engineering and Science, Vol. 39, No. 5, 1999, pp.849-854.

20. Doshi, J & Reneker, D.H., “Electrospinning Process and Applications of Electrospun Fibers,” Journal of Electrostatics, Vol. 35, No. 2-3,1995, pp.151-160.