生物碟片之分離定量功能與精確閥門設計

LabCD Fixed Quantity Separation Function and Precise Valve Design

趙健燁(Jan-ya Zhao)

指導教授 ： 王金樹 ； 林水泉

國立臺北科技大學/機電學院/製造科技研究所/碩士(2006年)

摘要

微流體技術在生物晶片中快速發展，其微量總體分析系統(Micro Total Analysis System)便是將生醫檢測上取樣、樣本傳輸、混合、分離及偵測等功能。流體分離定量與流體閥門設計為生物碟片設計之基礎且重要之設計，亦為本論文之重點。
本論文考慮行星角、閥門擴閥角、管內液膜張力對臨界轉速影響，且提出流體碟片之臨界轉速理論，其優於前人學者包括James Lee碟片臨界轉速公式，吾人所提出不須修正經驗式流體碟片之臨界轉速與實驗值約5%誤差，相當精確且實用性。由此理論公式吾人設計了四種具不同功能功能性定量流體碟片，包括流體分流定量碟片、平行定量碟片、依序定量碟片、二次樣品純化定量碟片，經實驗後其定量功能符合吾人所需功能。最後依流體碟片臨界轉速理論發展出以Visual Basic結合AutoCAD完成之分析軟體，使得微流體碟片閥門之設計與功能設計上可以快速自動化之設計。
最後吾人利用以上之碟片設計應用至尿液之檢測，利用影像處理判斷RGB值之檢測判別，成功地符合一般尿液檢測之八種功能，完成了一應用之實例，且吾人希望未來能結合生醫知識多方之應用，擴大LabCD之應用面。

關鍵字

微流體 ； 流體碟片 ； 臨界轉速 ； 定量分離

並列摘要

Micro-fluid techniques are developed rapidly in the bio-chip area. The Micro Total Analysis System provides the capabilities in sampling, transporting, mixing, separating, and sensing functions. The fluid separation technique and fluid valve design are the main developments in labCD research, and these are also main topics in this thesis.
This thesis proposed the critical rotation speeding theory which has better performance than the theory proposed by James Lee. We proposed an experimental function for the LabCD, and the error is around 5% comparing to the experiment result. We designed four chips for different functions, fundamental separation fixed quantity chip, parallel separation fixed quantity chip, series separation fixed quantity chip, and combinative separation fixed quantity chip. The experiment results satisfied what we expected. The software combing Visual Basic and AutoCAD is developed according to the critical velocity theory of the fluid chip.
Finally, we apply the method to inspect the urine, and the image processing technique is employed to process the RGB values. The experiment successfully satisfied the eight criteria of general urine inspection. We hope this research can be combined with more biomedical applications, and increase the applicability of LabCD.

並列關鍵字

Microfluid ； labCD ； critical rotation speeding ； Quantity

參考文獻

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