探討關節液生物分子於CoCrMo材料表面之接觸角與摩擦潤滑行為之關係

The Study of the Synovial Fluid Compositions the Relationship between Contact Angle on The Surface of CoCrMo Alloy Materials and Tribological Lubrication Behavior

練郁萍(Yu-Ping Lien)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/化學工程研究所/碩士(2008年)

https://doi.org/10.6841/NTUT.2008.00235

摘要

人工膝關節置換最大問題為人工膝關節植入物周圍產生骨溶解(osteolysis)的現象，當人工關節動作時不同關節液分子會造成不同的摩擦機制，所以我想要深入研究探討人工關節材料間因摩擦產生時，各關節液在其中所扮演的重要角色及物理性質。
本研究利用不同關節液白蛋白（albumin）、球蛋白（globulin）、玻尿酸(hyaluronic acid)及磷脂質(lipid)等單雙成分關節液進行接觸角測試。實驗配置的濃度為0.1mg/ml、 0.5mg/ml,、2.5mg/ml and 12.5mg/ml；至於双成分溶液則是額外再添加0.1 mg/ml的磷脂質。探討實驗所得到的接觸角與單、雙成份關節液分子在5MP的接觸壓力下的摩擦係數之關係。
由實驗數據可知，白蛋白與球蛋白隨濃度增加接觸角變小，對材料吸附力增加所以摩擦係數變大；玻尿酸與磷脂質隨濃度增加接觸角變大，對材料吸附力減弱，使兩對磨（sliding surfaces）之間潤滑液減少摩擦係數變大；0.1mg/ml磷脂質添加其他關節液中，發現雙成分溶液的接觸角與摩擦係數均大於單成分溶液的接觸角與摩擦係數，推測磷脂質先吸附在材料表面，另一端親水端與蛋白質緊密的黏著在一起，隨濃度增加於兩對磨間的吸附量變多，破壞磷脂質邊界潤滑薄膜，所以有較大的摩擦係數；玻尿酸與磷脂質因極性作用力及玻尿酸本身彈性(elastic property) 與黏性(viscous property) 兩種獨特的流變性質（rhelogical property）所致，隨濃度增加，接觸角與摩擦係數均變大，因此推論添加0.1mg/ml磷脂質並沒有達到增加潤滑效果。

關鍵字

接觸角 ； 白蛋白 ； 球蛋白 ； 玻尿酸 ； 磷脂質 ； 吸附力.

並列摘要

In medical procedure of “artificial knee joint replacement”, the most serious obstacle confronted is called “Osteolysis” (decade of surrounding tissues) . When artificial replacements are in movement, different composition molecules of different joint liquids will produce different friction phenomenon. Thus, the author wished to probe into crucial roles played by various liquid molecules in cases of friction, as well as identifying relevant physical properties thereof.
This study employed different joint liquids, the likes of: Albumin, Globulin, Hyaluronic Acid, Lipid, etc.; single & paired compositions tested from various contact angles. Various concentration levels of 0.1mg/ml, 0.5mg/ml, 2.5mg/ml, 12.5mg/ml were applied; for paired compositions, an extra 0.1 mg/ml of Lipid were added. Behavioral patterns in relation to “friction coefficients” obtained under contact pressure of 5MP, in aspects of contact angle, single/paired composition, particular joint liquid used, etc. were then further investigated.
Following observations could be made from laboratory test results:
Regarding single compositions: Both Albumin & Globulin showed diminished contact angles, with increase in concentration levels; hence, with stronger contact adhesion so increased friction coefficients. Whereas both Hyaluronic Acid & Lipid had wider contact angles with increase of concentration levels; with weaker contact adhesion which diminished lubrication between the “sliding surfaces”, hence increasing friction coefficients.Regarding paired compositions: By adding 0.1mg/ml of Lipid into different joint liquids, both the contact angles & the friction coefficients were greater than single compositions. The author presumes that, at one end when the Lipid is attached to the contact surface, the other end with hydrophilic and protein is “adhered to each other tightly”.Stronger adhesion showed between the sliding surfaces, with increased concentration level; hence, dissolving Lipid’s “boundary lubrication films”, hence, creating greater friction coefficients .Due to “polar interaction” derived from physical properties of both Hyaluronic Acid & Lipid, as well as “rhelogical properties” (e.g. elastic property & viscous property) of Hyaluronic Acid itself alone: With increase in concentration levels, both contact angles & friction coefficients increased too. The author presumes that, by adding 0.1mg/ml of Lipid, did not “positively” contribute, to the desired end-result of increased lubrication & prolonged life expectancy of artificial joints.

並列關鍵字

Contact Angle ； Albumin ； Lipid ； Globulin ； Adhesion .

參考文獻

蘇毓智. "生物潤滑液對超高分子量聚乙烯磨耗行為之影響. " 碩士論文, 國立北科技大學生物科技所, 台北, 2005年.

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