- 學位論文
使用金奈米粒子即時比色顯像偵測氟陰離子
A rapid colorimetric visualization assay of fluoride ion by using gold nanoparticles
摘要
對於開發生物陰離子檢測器一直令人有著高度的興趣，因為這些陰離子在一些生理學的過程中扮演很重要的角色，其中氟陰離子在健康與環境爭議上有著相當重要的地位，在此我們設計與合成出一種即時偵測氟陰離子的新型隱藏式奈米金聚集探針，可運用於建立微量簡易辨別氟離子平台，即時偵測環境危害物。利用氟離子脫去在保護在酚基上的矽基團，引發了探針分子內的1,6-重排脫去反應，隨即會放出會雙硫基直鏈分子，導致其自發性不可逆的聚集金奈米粒子，達到顯色偵測的目的。奈米金靈敏的反應性與聚集變色的特性來檢測在水溶液中的氟陰離子，此種檢測方法，有極佳的選擇性，可以在水溶液中使用不受干擾，而且還可以快速、裸眼來辨識低濃度(0.5mM)氟離子的存在，並且可以使用紫外線光譜儀簡易的達到定量效果。
並列摘要
The development of sensors for important biological anions is currently of great interest because of their indispensable roles in vital physiological process. Fluoride ion is one of the most attractive targets because of their considerable significance for health and environmental issues. Here, we have prepared a new switch-on latent AuNP agglomerization probe to detect fluoride ions in the presence of gold nanoparticles. The fluoride ion removes the silyl moiety on the phenol of the AuNP agglomerization probe followed by 1,6-rearrangement-elimination reaction and concomitant ejects the AuNP agglomerization dithiol linkers, which are spontaneous and irreversible at physiological temperatures in aqueous media. The AuNP agglomerization dithiol-modified flexible linker facilitates the aggregation of AuNP and leads to colorimetric change which is easily visualized by naked-eyes. The color generated by the cascade reactions was specific and insensitive to various anions. Furthermore, the assay platform developed herein was a sensitive colorimetric visualization assay for the quantitatively measurement of fluoride ion within micromolar range.