超音波及靜磁場對基因傳遞效率影響之研究-體外細胞實驗

The Effect of Ultrasound and Static Magnetic Fields on Gene Delivery Efficiency-An in Vitro Study

陳永裕(Yung-Yu Chen)

指導教授 ： 呂志誠

國立臺北科技大學/機電學院/機電整合研究所/碩士(2007年)

https://doi.org/10.6841/NTUT.2007.00202

摘要

本研究的目的首先在於探討由永久磁鐵產生，沒有電場作用的的靜磁場，對於增進DNA對細胞膜的穿透是否具有正面的效果；再者，對於超音波及磁場二種在傳統醫療應用上(如核磁共振(MRI)、超音波影像掃描等)被認為較安全及施用不具侵入性的能量，共同作用於細胞後對基因傳遞能否產生加成的效應。研究中將構建一組適用於體外(in vitro)細胞實驗的超音波系統，並利用銣-鐵-硼(Nd-Fe-B)永久磁鐵產生靜磁場環境，將pGL3 Luciferase報導基因傳遞至人類胚胎腎細胞(HEK293 cell)內，再利用冷光儀(Luminometer)觀察細胞中的Luciferase反應以評估傳遞效果。初步的實驗結果顯示，單獨僅有靜磁場的作用對基因傳遞後的表現並無明顯增強的效果，但是若配合超音波的作用，則顯示出了較單獨施用靜磁場或超音波時皆更佳的表現結果。實驗數據顯示，在最佳情況中超音波與靜磁場共同施用可較單獨施用超音波得到高出近7倍的表現結果。

關鍵字

超音波 ； 空腔效應 ； 靜磁場 ； 基因傳遞

並列摘要

The objectives of this study aim to probe, firstly, whether the bioeffects of a static magnetic field produced by permanent magnets are able to increase the permeability of cell membranes and thus enhance the gene delivery efficiency. Furthermore, the synthetic bioeffects of both static magnetic field and ultrasonic exposure regarded as safer and non-invasive operations, such as MRI and ultrasound scan, are preliminarily studied for elevating gene delivery efficiency. An ultrasound system was completed for in vitro gene delivery experiments, and added was the static magnetic field provided by Nd-Fe-B permanent magnets into experiment parameters. The pGL3-Luciferase reporter gene was delivered into the cultured HEK293 cell line, and detection of luciferase activity using the luminometer was performed. Our preliminary results reveal that sole static magnetic field exposure is unable to enhance transgene expression; however, the synthetic delivery efficiency induced by ultrasound and static magnetic fields is shown much better for the first time than either static magnetic field or ultrasound exposure only. In the best experimental case, the transgene expression with appropriately static magnetic exposure was significantly improved up to 7-fold high after sole ultrasound application.

並列關鍵字

ultrasound ； cavitation ； static magnetic field ； gene delivery

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