以浸鍍法於鈦金屬表面架構抗菌藥物之控制釋放機制

Establishment of Controlled Release Mechanism for Antibiotic on Titanium Surface by Dip-coating

吳益豪(Yi-Hao Wu)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/生物科技研究所/碩士(2010年)

https://doi.org/10.6841/NTUT.2010.00308

摘要

老年化社會是未來不可避免的趨勢，隨著老年人口愈來愈多，在金屬植入物的醫療器材需求日益急迫，以人工關節及為例，由於人工關節置換屬於侵入性手術，在手術後感染細菌的風險偏高。雖然目前病人術後可以口服抗菌藥物、塗藥膏或靜脈注射注射抗菌藥物，但這些方法仍有效果不佳及副作用的問題存在，抑菌效果嚴重不佳時甚至必須再次手術。
故本研究利用浸鍍法（dip-coating）塗佈含有健大黴素（gentamicin）的聚乙烯醇（PVA）在奈米鈦管表面形成薄膜，再將含有健大黴素的聚乳酸（PLA）微粒貼附於薄膜表面，達到二階段（two phases）藥物釋放，進而達到有效、長期的抑菌效果。
結果發現以15 wt％聚乙烯醇溶液濃度所製備的含藥聚乙烯醇薄膜能夠有較長期的藥物釋放效果，其中又以1.6 mm/s的拉起（pull-out）速度能夠製備最厚的薄層，製備薄層的聚乙烯醇溶液藥物濃度越高，在釋放初期也會得到較高的濃度；而含有健大黴素聚乳酸微粒之降解較緩慢而藥物釋放也較緩慢。再藉由細菌抑制環測試（bacterial inhibition zone test）來檢測含藥聚乙烯醇、含藥聚乳酸微粒及此兩者複合模式之抑菌效果。因此可以藉由改變聚合物重量百分濃度、藥物濃度以及拉起速度來調整未來符合體內有效、長期的抑菌效果。

關鍵字

浸鍍法 ； 健大黴素 ； 聚乙烯醇 ； 聚乳酸 ； 奈米鈦管 ； 細菌抑制環測試

並列摘要

Aging population society is the trend of the future. Hence there are more and more elders, who are in urgent need of metal implant, for example, the artificial joint. The artificial joint replacement is one kind of invasive operation, so the risk of the bacterial infection is so high after surgery. Although nowadays’ methods are that patients take a medicine by oral, apply the medical cream or treat a intravenous antibiotiotic therapy after the surgery, there are still some poor treatments and side-effects, and the once more operation is required if the antibacterial result is ineffective.
So this study was to apply dip-coating that coat titania nanotubues with gentamicin-contained poly vinyl alcohol (PVA) film, and established the combination model what is to make the gentamicin-loaded poly lactic acid (PLA) microspheres cling on the PVA film surface to achieve two phases drug release and then get the efficacious, long-term antibacterial effect.
Experimental results showed that the PVA films made by 15wt％ PVA solution could get the longer drug release effect, in addition, in this PVA concentration the thickest PVA film could made with the 1.6 mm/s start-up speed, and when using the higher drug concentration solution to forming the films, the higher primary drug concentration was released. By reason of the slower degradation time of gentamicin-loaded PLA, the drug was slower released. Finally applying the bacterial inhibition zone test to know the antibacterial effects of the drug-contained PVA films, the drug-loaded PLA microspheres and the combination model. Therefore, by adjusting the concentration of polymer solution, the amount of the drug and the start-up speed to satisfy the efficacious, long-term antibacterial effect in the body in the future.

並列關鍵字

Dip-coating ； Gentamicin ； Poly vinyl alcohol (PVA) ； Poly lactic acid (PLA) ； Titania nanotubues ； Bacterial inhibition zone test

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