組織再生膜之合成與動物實驗探討

Preparation and in vivo investigation of synthesized tissue regeneration membrane

張峻菱(Chun-Ling Chang)

指導教授 ： 鍾仁傑

國立臺北科技大學/工程學院/化學工程研究所/碩士(2011年)

摘要

本實驗以去免疫端的第一型膠原蛋白交聯製備膜片材料，使用低聚合原花青素作為交聯劑，評估此交聯膠原蛋白膜片作為引導組織再生膜的可行性。
經不同濃度原花青素處理後膠原蛋白膜片（OPCs-Col film）之機械強度達 30~60 kPa ，其熱穩定性高於未交聯之膠原蛋白薄膜，製備出的膠原蛋白膜可有效地抵抗膠原蛋白酶的分解達五十天。以材料強度、熱穩定性和抗酵素分解等實驗結果評估， 10% OPCs-Col film 為符合實驗目的材料。分析細胞實驗結果，10% OPCs-Col film 具有良好生物相容性。將材料植入活體，做大鼠皮下及頭蓋骨缺損癒合兩種動物實驗評估，結果顯示，於背部皮下測試中組織再生膜隨著植入時間約一個月後被降解吸收，而炎症反應也隨著植入時間增加而減輕；此組織再生膜對於大鼠腦組織並沒有造成任何病變產生，並可於材料內部觀察到新生骨。由動物實驗結果得知，此組織再生膜可幫助皮下組織修復，並延長植入活體之降解時間，達成阻隔生長較快速的上皮細胞及結締組織之增生，同時預留空間，以利生長速率較慢之骨細胞進行骨質新生。

關鍵字

第一型膠原蛋白 ； 低聚合原花青素 ； 引導組織再生膜 ； 細胞實驗 ； 動物實驗

並列摘要

In this study, type I collagen was crosslinked to prepare as membrane through using oligomeric proanthocyanidins（OPCs）as the crosslinking agent. The fabricated materials were evaluated to be applied as guided tissue regeneration membranes.
The mechanical strength of the crosslinked collagen membrane using different concentration of oligomeric proanthocyanidins ranged from 30 to 60 kPa. The crosslinked collagen membranes had better thermal stability than uncross-linked ones and could effectively resist the decomposition in collagenase solution as long as fifty days. The results of material analyzing showed that 10% OPCs-Col film was ideal for our purpose. In vitro study revealed that 10% OPCs-Col film had great biocompatibility. The material was further severed for in vivo studies with two models, subcutaneous and cranium defect in rat. The subcutaneous test showed that the regeneration membrane was degraded till one month and the inflammatory response also reduced with implantation time. When implanted into the cranium defect, no lesions of the brain were caused and new bone tissue was observed inside the material. The results of in vivo studies showed that the synthesized membrane was helpful for tissue regeneration with long degradation time. The tissue regeneration membranes can barrier the rapid growing soft tissue, in order to save the capacity for the growth of neo bone.

並列關鍵字

Type I Collagen ； Oligomeric Proanthocyanidins ； Guided Tissue Regeneration membrane ； In vitro Study ； In vivo Study

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