The function of permeability guided tissue regeneration membrane is to enhance tissue regeneration of periodontium by preventing gingival epithelium and connective tissue barrier adverse effect. The healing process can be confirmed by histological studies – regeneration of functional peridonium occurs only as the connection of tissue periodonium to root surface. Pore size, thickness, strength of regeneration membrane, as well as it physical-chemical quality of material (biocompatibility) will affect the successful of guided tissue regeneration directly. So, it become a very important issue to find out a method for controlling the pore size, structure and physical-chemical quality of regeneration membrane.