利用電化學陽極處理方式進行鈦金屬表面改質並評估其促進骨整 合之效應

Enhancement of osseointegration on titanium surface by anodizing process

李欣純(Hsin-Chun Li)

指導教授 ： 方旭偉

國立臺北科技大學/工程學院/化學工程研究所/碩士(2009年)

摘要

本研究主要是利用電化學陽極處理法將鈦金屬進行表面改質而形成氧化鈦奈米管，並藉由改變不同的實驗參數，如陽極處理電壓、時間和陽極旋轉等變因，分別觀察對於其結構上的影響。實驗結果顯示，當電壓逐漸升高時，氧化鈦奈米管的成長速度、長度和孔徑大小逐漸的增加。而加裝馬達使陽極旋轉進行陽極處理時，隨著時間的增加，可發現到奈米管會發生破裂因而形成奈米線或奈米帶的結構。 由於鈦金屬表面形貌的不同，將會對骨細胞的生長有著極大的影響，因此本實驗進行類骨母細胞(osteoblast-like cell) MG-63培養於氧化鈦奈米管及氧化鈦奈米線兩種不同的表面形貌，並於特定的時間點分別對細胞的貼附，增殖作不 同的測試，並加以比較不同條件下這些測試的結果。 研究結果顯示，類骨母細胞經24小時培養於氧化鈦奈米線上有著較佳的貼附率;骨細胞的形態已呈現明顯伸展的外貌，且細胞的增值及分化都有較良好的表現。

關鍵字

鈦金屬 ； 陽極處理法 ； 氧化鈦奈米管 ； 類骨母細胞 ； 骨整合

並列摘要

In this work, titania nanotubular were fabricated by anodizing technique. Effects of anodization condition such as the applied voltage, anodizing time and rotated the anode were investigated. It was found that, the growth rate, length and pore diameter is depending on the applied voltage. When the anode to rotate, the titanium nanotubes readily causing crack of nanotubes and turns into the structure of nanowire or nanobelt.
The surface properties of titanium plays an important role in cell morphology and behaviors such as cell adhesion, migration, proliferation and differentiation. We seeded the Osteoblast-like cells on three different morphology of titania film ( flat, nanotubes and nanowires). The results indicated that the titania film with nanowires yield the best biological effects for cell adhesion and proliferation.

並列關鍵字

Titanium ； anodic ； titanium oxide nanotubes ； osteoblast-like cell ； osseointegration

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