靜電紡絲技術製備水溶性幾丁聚醣/聚乙烯醇奈米纖維薄膜於抗菌之應用

Electrospinning of sulfonated chitosan/ polyvinyl alcohol nanofibrous membrane and application of antibacterial

洪琇議(Hsui-Yi Hung)

指導教授 ： 芮祥鵬

國立臺北科技大學/工程學院/有機高分子研究所/碩士(2009年)

摘要

幾丁聚醣(Chitosan)是一生物可分解性材料，具有良好的生物相容性及物理化學性質，可應用於生醫材料方面，將其製成纖維膜，使其應用範圍更廣泛。本研究經由化學修飾法來製造磺酸化幾丁聚醣(sulfonated chitosan)，改質後的幾丁聚醣因為具有親水性的磺酸基，可提高幾丁聚醣的溶解性質。
靜電紡絲技術是近年新穎的奈米科技，其可以快速且直接將高分子材料轉變成奈米纖維。本研究是以靜電紡絲的方式，以聚乙烯醇及水溶性幾丁聚醣這二種高分子為材料，水為溶劑，將其做為奈米纖維薄膜。以10%PVA和1~3wt% sulfonated chitosan摻合
，在電壓15kV，距離10cm為最佳參數製備奈米纖維膜，平均纖維直徑約200~390nm。並將其應用於抗菌方面，對金黃色葡萄球菌之抗菌能力測試結果發現，水溶性幾丁聚醣/聚乙烯醇纖維膜中，含有16.67%的水溶性幾丁聚醣，對金黃色葡萄球菌就有抗菌的效果，抗菌率為99.91%。
最後，我們以戊二醛蒸氣當交聯劑使其形成水溶性幾丁聚醣/聚乙烯醇網狀結構纖維膜。從TGA、DSC分析結果證明，交聯後的水溶性幾丁聚醣/聚乙烯醇纖維膜可以增加結構的熱安定性進一步也增加了主鏈結構的熱穩定性及提高玻璃轉移溫度；DMA分析結果顯示，交聯後纖維膜的剛性增加，其應力提升而應變下降。

關鍵字

靜電紡絲 ； 聚乙烯醇 ； 水溶性幾丁聚醣 ； 交聯 ； 抗菌

並列摘要

Chitosan is a biodegradable material that has attracted enormous attention in agricultural, pharmaceutical and medical industry due to its good biocompatibility and excellent physical and chemical properties. The purpose of this research is to modify chitosan structure via a chemical method. The modified chitosan has sulfonic acid group, the solubility of chitosan in water is significantly improved.Electrospinning has been recognized as an efficient technique for the fabrication of polymer nanofibers and various polymers have been successfully electrospun into ultrafine fibers during the last few years. Composite nanofiber membranes of sulfonated chitosan and PVA were the materials using water as a solvent by electrospinning in this study as an nanofiber membrane. Non-beaded and uniform nanofibers from the following optimum condition:10%PVA solution with 1~3wt% sulfonated chitosan was successfully electrospun at 15 kV operating voltage and nozzle to collector distance of 10cm, and application of antibacterial. The results, it showed that sulfonated chitosan/PVA membranes containing 2wt% sulfonated chitosan could inhibit the growth of staphylococcus.
Finally, the sulfonated chitosan is crosslinked with glutaraldehyed to form membranes of networks. In the TGA、DSC analysis demonstrated that the nanofiber membranes of sulfonated chitosan/PVA membranes of sulfonated chitosan networks can increase sulfonic acid group in the structure are thermal stability and the glass transition temperature .In the mechanical properties, the result indicated that cross-linking with glutaraldehyde improves stress and decreases strain of the nanofiber membrane.

並列關鍵字

Electrospinning ； polyvinyl alcohol ； Sulfonated chitosan ； Cross-linking ； antibacterial

參考文獻

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