銀添加對生醫陶瓷之結構、物理特性與體外抗菌之影響

Influence of silver addition on microstructure, physical properties and in vitro antibacterial of biomedical ceramics

王傳鑫(CHUAN-HSING WANG)

指導教授 ： 黃瓊芳

臺北醫學大學/口腔醫學院/醫療器材產業碩士專班/碩士(2013年)

https://doi.org/10.6831/TMU.2013.00138

摘要

本研究主要觀察含銀之氧化鋯複合材料之奈米銀顆粒對生物膜形成的影響。依據標準的實驗流程，JIS Z2801：2010和ATCC6538P規範進行抗菌試驗，在此使用革蘭氏陽性菌金黃色葡萄球菌（S. aureus）和革蘭氏陰性菌的大腸桿菌（E.coli）的。結果顯示，氧化鋯與銀除了具有優異的抑制性能，對金黃色葡萄球菌和大腸桿菌生物膜的形成。此外，Ag相的析出物在抗菌的機制中發揮重要的作用。釋放的Ag相析出的銀離子有效殺死細菌，破壞其細胞壁和細胞膜，造成其細胞質外流。含有Ag之生物醫學氧化鋯之複合材料，可以作為生物材料或醫療用，廚房和食品加工器具之抗菌材料。

關鍵字

氧化鋯 ； 抗菌 ； 銀 ； 生物膜

並列摘要

This study investigated the influence of nano-silver particle on biofilm formation for the Ag-containing zirconium oxide composites. A standard experimental procedure JIS Z2801:2010 and ATCC6538P specification were performed in this antibacterial test by using Gram-positive bacterial Staphylococcus aureus (S. aureus) and Gram-negative bacteria Escherichia coli (E. coli). Results show that the zirconium oxide with silver addition exhibited excellent inhibition properties against the biofilm formation of S. aureus and E. coli. Furthermore, the Ag phase precipitates play an important role in the antibacterial mechanisms. The Ag+ ions released from the Ag phase precipitates efficiently kill bacteria by destroying their cell walls and cell membranes and causing their cytoplasm to leak. The Ag-containing biomedical zirconium oxide composite can be considered as a candidate antibacterial material for use as a biomaterial or in hospitals, kitchens, and food-preparation appliances.

並列關鍵字

Zirconium oxide ； Antibacterial ； Silver ； Biofilm

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