高劑量β－胡蘿蔔素在攝取不同油脂大白鼠體內之利用率及對脂質代謝的影響

The availability and effects on lipid metabolism of large dose β-carotene in rats fed different fat diets.

楊維祥(Yang Wei-Shiang)

指導教授 ： 謝明哲 ； 鄭心嫻

臺北醫學大學/藥學院/藥學研究所/碩士(1993年)

摘要

本研究的目的是探討高劑量 β-胡蘿蔔素在攝取不同油脂大白鼠體內之利 用率，進而研究 β-胡蘿蔔素在脂質代謝上所扮演的角色。將80隻 Wistar品系雄性大白鼠(重約 250公克) ，隨機分成八組，以 β-胡蘿蔔 素 (0.2 ％ ，給予或不給予)、脂質種類 ( 黃豆油或豬油 )及脂質濃 度(5 ％或15 ％) 作 2×2×2 factorial的設計，於第3週及第6週分別宰 殺大白鼠，取血液及肝臟，測定血清與肝臟中三酸甘油酯及總膽固醇含量 ，血清中並分析低密度脂蛋白膽固醇及高密度脂蛋白膽固醇含量，同時利 用 HPLC分析血清及肝臟中β-胡蘿蔔素、維生素 A含量。結果顯示：食物 攝取量無顯著差異；不論是否給予 β-胡蘿蔔素，高黃豆油組有顯著較高 的最終體重及餵食效率( P <0.01)。脂質分析方面，黃豆油／給予β-胡 蘿蔔素組之血清三酸甘油酯高於黃豆油／不給予β-胡蘿蔔素組，豬油組 有相反的結果( P=0.0007)；第3週之肝臟三酸甘油酯，高黃豆油組顯著高 於其它組 (P<0.05)，而第 6 週發現給予 β-胡蘿蔔素會降低肝臟三酸甘 油酯 (P=0.0051) ；血清中總膽固醇、高密度脂蛋白膽固醇及低密度脂蛋 白膽固醇，於第 3週無顯著差異，第6週則是高油組高於低油組(P<0.05) ，且血清β-胡蘿蔔素濃度與高密度脂蛋白膽固醇／低密度脂蛋白膽固醇 比率呈負相關 (r=-0.35，P=0.0374)。第 3週之肝臟膽固醇在高油時，黃 豆油組高於豬油組，低油時則相反 (P=0.0001)，第6週時，高黃豆油組顯 著高於其它組(P <0.005)。血清及肝臟中 β-胡蘿蔔素有相同的趨勢， 第 3週時，高油組顯著高於低油組( 血清，P=0.0028；肝臟，P=0.0001) ，且高豬油組高於高黃豆油組(p<0.05)。β-胡蘿蔔素的給予對於血清維 生素 A在第3週時無影響，第6週時則會降低；肝臟維生素A則會因β-胡蘿 蔔素的給予而上升。

關鍵字

油脂 ； β-胡蘿蔔素 ； 三酸甘油酯 ； 膽固醇 ； 維生素A

並列摘要

The purpose of this study was to investigate the availability and the effects on lipid metabolism of large dose β-carotene (BC) in rats fed different fat diets. Male adult Wistar rats( N=88 ) were randomly divided into eight groups in a ２×２×２ factorial design for three or six weeks. These diets varied in BC content (0.2 ％, with or without), lipid type (soybean oil or lard) and lipid level (5 ％ or 15 ％). Analytical items included serum tri- glyceride(STG), serum total cholesterol( SC), high density lipo- protein cholesterol(HDL-C), low density lipoprotein cholesterol (LDL-C), liver triglyceride(LTG) and liver total cholesterol(LC). HPLC was used to analyze BC and vitamin A concentrations. The re- sults showed that 15 ％ soybean oil group had higher final body weight and feed efficiency (p<0.0100). The effects on lipid meta- bolism were as follows: soybean oil/with BC group had higher STG than soybean oil/without BC group,and the lard groups had reverse result (p=0.0007); 15％ soybean oil group had highest LTG amomg eight groups after three-week feeding, and it was lowest when given BC after six-week feeding (p=0.0051); 15％ oil/fat groups had higher SC, HDL-C and LDL-C than 5％ oil/fat groups after six- week feeding (p<0.05), in addition, serum BC had negative correl- ation (r=-0.35, p=0.0374) with HDL-C/LDL-C ratio; 15％ soybean oil group had higher LC than 15％ lard group after three-week feeding, 5％ oil/fat groups had reverse result (p= 0.0001), but 15％ soybean oil group had highest LC after six- week feeding (p< 0.005). Serum and liver BC showed similar results that 15％ oil/ fat groups had higher BC than 5％ oil/ fat groups (serum, p=0.0028 ; liver, p=0.0001), and 15％ lard group had higher BC than 15％salad oil group (p<0.05). With BC groups had lower serum vitamin A than without BC groups after six-week feeding. Liver vitamin A increased with the presence of BC (p<0.05).

並列關鍵字

Fat ； β-carotene ； Triglyceride ； Cholesterol ； Vitamin A