靈芝之成分研究

王秀枝

指導教授 ： 徐鳳麟

臺北醫學大學/藥學院/藥學研究所/碩士(2002年)

摘要

中文摘要 靈芝是一種著名的中國草藥，在亞洲靈芝已廣泛地被使用於保健以及延年益壽。近年來，亞太各國已把焦點放在中草藥的發展研究，而靈芝是其中之一。靈芝含有多種化學成分，其相關的藥理作用雖已被廣泛探討，但市售產品非常混亂，在品質管制方面缺乏可供作指標成分者，因此，本論文對靈芝所含之化學成分作進一步的研究，以便作為靈芝製備之參考化合物。 靈芝之子實體在室溫時以甲醇萃取，經過各種層析管柱分離及純化分離後，初步得到九個化合物，其結構經由光譜分析鑑定之後，確認為 Ganodermic acid G (1), Lucidone A (2), Pachymic acid (3), Polyporenic acid C (4), Stellasterol (5), Pterocarpin (6), Trifolirhizin (7), Kuraridinol (8), Kuraridin (9)。

關鍵字

靈芝 ； 層析管柱 ； 光譜分析

並列摘要

Abstract Ganoderma lucidum (Fr.) karst ( polyporaceae ) is a well-known Chinese crude drug which has been widely used for promotion of health and longevity in East Asia. Current trend of phytotherapy research prevailing in Asia pacific country, Ganoderma lucidum is one of the focal point in the development of Chinese herbs. Ganoderma lucidum contains several chemical constituents, which have been investigated in relation to their physiological effects. The compounds can be used the standards in QC and the reference constituents in preparation of standards. Therefore, a chemical investigation of compounds in Ganoderma lucidum was performed. The fruit body of Ganoderma lucidum was extracted with MeOH at room temperature. By means of various chromatographic methods, nine compound were isolated and purified. On the basis of spectral analysis, the structure of this compound was determined to be Ganodermic acid G (1), Lucidone A (2), Pachymic acid (3), Polyporenic acid C (4), Stellasterol (5), Pterocarpin (6), Trifolirhizin (7), Kuraridinol (8), Kuraridin (9)。

參考文獻

1. Wu, T. S.; Shi, L. S.; Kuo, S. C. Cytotoxicity of Ganoderma lucidum triterpenes. J. Nat. Prod. 2001, 14, 1121-1122.

2. Bao, X.; Liu, C.; Fang, J.; Li, X. Structural and immunological syudies of a major polysaccharide from spores of Ganoderma lucidum (Fr.) Karst. Carbohydr. Res. 2001, 332, 67-74.

3. Min, B. S.; Gao, J. J.; Nakamura, N.; Hattori, M. Triterpenes from the spores of Ganoderma lucidum and their cytotoxicity against Meth-A and LLC tumor cells. Chem. Pharm. Bull. 2000, 48 (7), 1026-1033.

4. Min, B. S.; Nakamura, N.; Miyashiro, H.; Bae, K. W.; Hattori, M. triterpenes from the spores of Ganoderma lucidum and their inhibitory activity against HIV-1 protease. Chem. Pharm. Bull. 1998, 46 (10), 1607-1612.

5. Wang, S. Y.; Hsu, M. L.; Hsu, H. C.; Tzeng, C. H.; Lee, S. S.; Shiao, M. S.; Ho, C. K. The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes. Int. J. Cancer. 1997, 70, 699-705.