米麩油的攝取對STZ誘導糖尿病大白鼠血糖控制及脂質代謝之影響

Effects of rice bran oil on glycemic control and lipids metabolism in STZ-induced diabetic rats

鄭惠如(Huei-Ju Cheng)

指導教授 ： 鄭心嫻 博士

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2004年)

摘要

本次研究目的在探討米麩油的攝取下，對糖尿病大白鼠體內血糖控制及脂質代謝之影響。實驗採用40隻10週齡Wistar品系雄鼠，分別為20隻經由腹腔注射 nicotinamide (200 mg/kg BW)及streptozotocin (STZ, 45 ~ 40 mg/kg BW) 誘導糖尿病大白鼠及20隻正常血糖大白鼠，進行四週實驗，以AIN-76標準配方調配實驗飼料。實驗共分成四組為大豆油正常組(SN)、米麩油正常組(RN)、大豆油糖尿病組(SD)、米麩油糖尿病組(RD)。實驗開始、第二週及第四週採取禁食血液樣本，實驗結束時犧牲大白鼠取其肝臟及副睪脂肪組織。檢測項目包括：禁食血漿葡萄糖、胰島素、果糖胺、三酸甘油酯、游離脂肪酸、總膽固醇、低密度脂蛋白膽固醇、高密度脂蛋白膽固醇濃度及脂肪酸組成；肝臟三酸甘油酯、總膽固醇、脂質過氧化物MDA濃度及脂肪酸組成。結果顯示，此次STZ誘導糖尿病模式能引起糖尿病性高脂血症。四週實驗後，在血糖方面，RD血漿葡萄糖及果糖胺濃度顯著低於SD組(P=0.0071) (P<0.0001)而胰島素濃度變化不顯著。在脂質方面，RD組血漿三酸甘油酯、總膽固醇及低密度脂蛋白膽固醇濃度顯著低於SD組(三者皆為P<0.0001)且RD組血漿高密度脂蛋白膽固醇濃度顯著高於SD組(P<0.0001)。RN組血漿三酸甘油酯、總膽固醇及低密度脂蛋白膽固醇濃度顯著低於SN組(三者皆為P<0.0001)且RN組血漿高密度脂蛋白膽固醇濃度顯著高於SN組(P<0.0001)。RD組肝臟三酸甘油酯濃度顯著低於SD組(P=0.0235)。在血漿脂肪酸方面，RD組18:1、總單元不飽和脂肪酸、20:4及22:6顯著高於SD組(P<0.05)；RN組16:1及18:1顯著高於SN組(P<0.05)。而餵食米麩油飼料組大白鼠體內18:2、18:3及總多元不飽和脂肪酸顯著低於餵食大豆油飼料組大白鼠(分別為P<0.0001, P=0.0144及0.0002)。RN組肝臟中18:1顯著高於其他各組，而RD組肝臟中22:6顯著高於其他各組。總結，米麩油的攝取對正常或糖尿病大白鼠而言，能降低血漿三酸甘油酯、總膽固醇及低密度脂蛋白膽固醇濃度及增加高密度脂蛋白膽固醇濃度，並降低體內18:2、18:3及總多元不飽和脂肪酸含量；對糖尿病大白鼠而言，能改善血糖控制及降低肝臟三酸甘油酯濃度。

關鍵字

米麩油 ； STZ誘導糖尿病大白鼠 ； 脂質代謝

並列摘要

This study was designed to investigate the effects of rice bran oil (RBO) on glycemic control and lipid metabolism in STZ-induced diabetic rats. Diabetes was induced by nictotinamide and streptozotocin injection. The formulas of these experimental diets were modified from the AIN-76 formula. Forty 10-week old male Wistar rats were divided into four groups: a normal rat group fed soybean oil (SBO)-containing diet (SN) or RBO-containing diet (RN) and a diabetic rat group fed SBO-containing diet (SD) or RBO-containing diet (RD). Fasting blood sampling was conducted at zero time, and at the end of the second and the fourth week in this experiment. At the end of this experiment, all rats were sacrificed, and their livers and epididymal fat pads were collected. In this study, STZ-induced diabetic rats showed hyperlipidemia. Whether normal or diabetic rats fed the RBO-containing diet group, the plasma total cholesterol, triglycerides and low-density lipoprotein cholesterol were significantly lower compared with these fed SBO-containing diet group at week 4 (RD < SD and RN < SN)；(P<0.0001). As to plasma high-density lipoprotein cholesterol, RD and RN were significantly higher compared with SD and SN, respectively (P<0.0001). Liver triglycerides were reduced in RD compared with SD (P=0.0235). Whether normal or diabetic rats fed the RBO-containing diet group, the 18:2, 18:3 and total polyunsaturated fatty acids (PUFAs) levels as percentages of total plasma fatty acids were significantly lower comparison with fed the SBO-containing diet group (RD < SD and RN < SN)(P<0.05). In conclusion, consumption of RBO could reduce plasma TG, TC, LDL-C, 18:2, 18:3 and total PUFA levels and increase HDL-C concentrations in normal and diabetic rats. In diabetic rats, consumption of RBO could improve glycemic control and decrease liver TG concentrations.

並列關鍵字

rice bran oil ； STZ-induced diabetic rat ； lipid metabolism

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