注射式長效抑菌性之引導組織再生阻隔材研究

Long-lasting Anti-bacterial Injectable Guided Tissue Regeneration Barriers

廖啟宏(Chi-Hung Laio)

指導教授 ： 楊正昌

臺北醫學大學/口腔學院/口腔科學研究所/碩士(2006年)

摘要

對於牙周病臨床治療而言，感染的預防扮演著極重要角色，本研究目的係以聚乳酸為基材，並導入幾丁聚醣，以開發出具有長效抑菌效果的可注射式引導組織再生材（Guided tissue regeneration barriers, GTR barriers），實驗顯示Chitosan在PLA溶液中最高含量為0.08 wt%。在對Actinobacillus actinomycetemcomitan之抑菌測試結果，額外添加2 wt%幾丁聚醣粉末於PLA/Chitosan/ACP barriers中，在第八天後抑菌效果比含有5 wt% Doxycycline高出約2.25倍，並於十八天後仍可維持56.58 %的抑菌效果。另外，以聚乳酸/幾丁聚醣作為基材並添加不同比例的非晶型磷酸鈣，經過水洗固化後可得白色片狀沉澱物，於室溫下靜置48小時後，複合材之體積呈現膨脹且具有可塑形之特性，以熱重分析儀與流變儀分析樣品之含水率與屈伏應力，結果顯示不同ACP含量對複合材含水率與屈伏應力具有消長變化，而PLA/Chitosan/ACP之可塑程度與其所吸收之水份多寡有關，推測可能機制為ACP磷酸鈣鹽解離後之金屬離子與幾丁聚醣之胺基透過螯合作用或靜電吸引之交互作用，形成具吸水性之離子團簇（Ionic cluster）分散於疏水性之聚乳酸基材中。

關鍵字

聚乳酸 ； 幾丁聚醣 ； 非晶型磷酸鈣 ； 引導組織再生材 ； 可注射式

並列摘要

Inflammation inhibition played an important role in periodontal treatment. The purpose of this study is to develop an long-lasting anti-bacterial injectable Guided Tissue Regeneration barriers (GTR barriers) compling antibacterial effect by acceding chitosan and ACP to PLA. The highest solubility of chitosan was about 0.08 wt %.The result of antibacterial test for Actinobacillus actinomycetemcomitan revealed that the antibacterial ability was 2.25 times higher when adding another 2wt% chitosan to the PLA/Chitosan/ACP barriers, compared to 5wt % Doxycycline after the eighth day. And the PLA/Chitosan/ACP barriers with additional 2 wt% chitosan still maintain 56.58 % antibacterial ability after eightieth day. The different composition of PLA/Chitosan/ACP composites was prepared by water washing solidification from NMP/water/lactic acid consolvent. After storing at room temperature for 48 hours and taking it out to obtain a shapable composites. To investigate the influence of ratio of composition with the shapable property of composites, it was analyzed by Rheometer, TGA, AFM, XRD. The result of the different APC content had different effect both water content and yield stress of composite materials. The yield stress decreased with ACP content while increases. It was derived that ions, released from ACP interact with amide groups of chitosan to form water-absorbing ionic clusters through chelating or electrostatic attraction.

並列關鍵字

Poly(lactic acid) ； Chitosan ； Amorphous calcium phosphate ； Guided tissue regeneration barriers ； Injecable

參考文獻

20.蔡尚文, 黎永康. 引導組織再生術治療上顎第一大臼齒之第三級根叉侵犯病例報告. 中華牙周醫誌. 2: 145-151, 1997.

1.Topic B. Classification of periodontal diseases. Int Dent J. 40: 171-175, 1990.

3.Gibbons RJ. Bacterial adhesion to oral tissues: a model for infectious diseases. J Dent Res. 68: 750-760, 1989.

4.Loesche WJ, Syed SA, Schmidt E, Morrison EC. Bacterial profiles of subgingival plaques in periodontitis. J Periodontol. 56: 447-456, 1985.

5.Moore WE. Microbiology of periodontal disease. J Periodontal Res. 22: 335-341, 1987.