氧氣電漿處理後鈦金屬植體的血液相容性之影響研究

Effect of oxygen plasma treatment on hemocompatability of titanium-based implant

蔡秉璋(Ping-Chang Tsai)

指導教授 ： 歐耿良 博士 ； 李勝揚 博士

臺北醫學大學/口腔學院/口腔科學研究所/碩士(2006年)

摘要

植入性生物材料與宿主組織接觸面的生物相容性對於骨整合的效果扮演著關鍵性角色。近年來有關植體表面改質的方式已漸漸朝向利用生物化學的方法來改善植體表面的生物活性，亦即控制特定生物分子的連結並減少非特定蛋白質的吸附來提升骨癒合的效果。其中利用低溫電漿(glow discharge)來活化鈦金屬表面進而連接特定蛋白或高分子基團於植體表面的應用已是十分普遍。本研究先利用氧氣電漿處理來產生一層緻密的二氧化鈦(TiO2)氧化層，提高鈦植體的抗蝕性避免離子的釋出並促進植體植入後跟骨組織間傷口的凝血及癒合。並利用丙烯胺的電漿處理在鈦金屬表面鍵結上胺基(-NH2)的官能基，之後再以交鏈劑戊二醛(glutaraldehyde)來固定生物活性膠原蛋白於低溫電漿處理後的鈦金屬表面。 以接觸角量測儀分析鈦試片改質後表面親疏水性的表面能變化。以X光光電子能譜儀(XPS)分析膠原蛋白吸附於鈦金屬後其化學鍵結的改變;以拉曼光譜儀(Raman)來探討低溫電漿處理的氧化層結構特性，再利用穿透式電子顯微鏡(TEM)來觀察植體與組織間界面的變化。藉由clotting time的測試來驗證此二氧化鈦層的血液相容性具有促進組織癒合能力，進而改善鈦植體與骨之間的骨整合作用。實驗結果顯示經由氧氣電漿處理後在鈦片上會生成一層厚度約為150nm且較為縝密的氧化層。此一氧化層可提升表面的親水性以及提高膠原蛋白的固定效果且擁有較快的血液凝結特性，進而能夠進一步改善骨組織的癒合能力及促進骨整合。希望透過以上實驗可提供一個能穩定鍵結生物活性分子到鈦金屬表面的方式，以期植體植入後能引導及促進組織復原。

關鍵字

低溫電漿 ； 二氧化鈦 ； 血液相容性 ； 骨整合

並列摘要

The biocompatibility of biomaterial surface direct contact with the host tissue plays a critical role in implantation. Biochemical method is a novel methodology with great potential for controlling initial bone-implant interactions in recently research. In order to improve the integration of implants, it is desirable to control interfacial reactions such that nonspecific adsorption of proteins is minimized to control and enhance tissue-healing phenomena. First, a dense TiO2 layer could be formed on titanium substrate by oxygen plasma treatment in this study. The oxide layer can prevent metal ion releasing and promotes blood coagulation to accelerate wound healing after implantation. Otherwise, an intermediary allylamine deposition was covalently linked to the oxide layer by glow discharge, the covalent binding of bioactive protein to the free terminal NH2 groups using glutaraldehyde as a coupling agent was followed. The contact angle was used to analyze the hydrophilic and surface energy of titanium oxides. The covalent immobilization of bioactive organic molecules and in vitro bioactivity were evaluated by Raman spectroscopy, x-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM). The hemocompatibility of titanium oxides were verified by clotting time assay. The result showed that a denser titanium oxide layer with about 150 nm thickness existed on the plasma treatment surface. The denser oxide layer by O2 plasma treatment performed the better surface wettability and enhanced the protein immobilization to have a better result in allylamine plasma treated plates and promoted initial blood coagulation. It is believed that nanostructural titanium oxide films can improve the tissue healing and then promote the osseointergration.

並列關鍵字

glow discharge ； rutile ； hemocompatibility ； osseointegration

參考文獻

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