骨再生法治療退化性骨骼病變之研究

Regenerative Approaches to Treat Degenerative Skeletal disease

劉明哲(Ming-che Liu)

指導教授 ： 鄧文炳

臺北醫學大學/口腔醫學院/牙醫學系碩博士班/博士(2013年)

摘要

骨質疏鬆症是一種疾病狀況，骨重塑時成骨細胞和蝕骨細胞之間的平衡被破壞，骨質密度（ BMD）的降低，造成腰椎和椎間盤的形態改變。第一部分的研究是建立一個模型篩選、發現和培養再生的藥物。不朽化人體髓核（ ihNP ）細胞株，用HPV-16 E6/E7逆轉錄病毒載體感染人體椎間盤的髓核細胞後建立。接著以軟骨形成的特定屬性及ihNP株細胞的再生潛能的2D和3D退化模型進行驗證。結果證實ihNP細胞具有成為治療軟骨細胞再生模式的潛力，並且用於一個功能強大的平台作為調查對NP細胞再生機制或藥物篩選。本文應用涉及的因素在骨形成，以評估海洋深層水（ DSW）在骨質疏鬆症的研究。本文第二部分為預防和治療骨質疏鬆的方法。通過使用以前建立的方法評估骨重建的過程中海洋深層水（ DSW）對治療骨質疏鬆的效用，本論文的研究發現海洋深層水（ DSW）在骨再生和骨量有恢復的效果。結果顯示， DSW顯著促進了細胞增殖活性和細胞聚落形成能力，增加成骨細胞和骨髓細胞（BMSCs ）。 DSW也誘導骨的再生，在骨質疏鬆小鼠恢復骨質量。

關鍵字

脊椎 ； 退化性骨骼病變 ； 再生法治療

並列摘要

Osteoporosis is a condition in which the balance of bone remodeling between osteoblasts and osteoclasts disrupted, bone mineral density (BMD) decreases, and the morphology of the lumbar spine and intervertebral discs changed. The first part research was to develop a model for screening, discovering and developing disc regenerative drug. An immortalized human nucleus pulposus (ihNP) cell line was established by using HPV-16 E6/E7 retroviral vector to infect NP cells of human IVD. Following the chondrogenic-specific properties were identified, the regenerative potential of ihNP cell line was verified in 2D and 3D degenerative models. The results implicated that the ihNP cell line has therapeutic potential for chondrocyte regeneration and can be used a powerful platform for investigating the NP regenerative mechanisms or drug screening. This thesis applied an approach involving factors in bone formation to evaluate the therapeutic potential of deep sea water (DSW) in the prevention and treatment of osteoporosis in the second part of study. Through the use of previously established methods evaluating the process of bone remodeling, the work of this thesis investigated the effectiveness of DSW in bone regeneration and bone mass recovery. The results indicated that DSW significantly promoted the proliferative activity and colony-forming ability, increased the expression of osteogenic markers and elevated matrix mineralization of bone marrow cells (BMSCs). DSW also induced the regeneration of bone and restoration of bone mass in osteoporostic mice.

並列關鍵字

spine ； degenerative Skeletal disease ； Regenerative approach

參考文獻

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