鈦金屬表面形貌對骨母細胞型態之影響

The effect of Ti surface morphology on the adhesion behavior of osteoblast-like cells

謝宗龍(Tsung-Lung Hsieh)

指導教授 ： 施永勳

臺北醫學大學/口腔醫學院/醫療器材產業碩士專班/碩士(2014年)

摘要

體外細胞反應被認為與基質的物理和化學性質有關聯。本研究中對不同化學組成和表面型態基質的鈦金屬表面進行MG- 63成骨細胞貼附評估，針對在鈦金屬表面不同處理:機械加工的孔洞與凹槽（control）、陽極氧化處理（AO）和陽極氧化伴隨水熱處理（HYT）三種類型。細胞貼附在經陽極氧化處理和陽極氧化伴隨水熱處理的表面於5小時後展現良好的伸展特性，將歸因於其化學因素，例如:
在表面包含鈣和磷。經24小時後，細胞完整平貼在經陽極氧化伴隨水熱處理的表面，但在只經陽極氧化處理的表面則沒有此效果，這說明提高細胞貼附行為還有物理因素，於特定表面形貌提供了細胞比較大的機械性刺激。進一步利用有限元方法評估細胞的應力分佈（P > 0.01 ），根據數據說明，對於經陽極氧化伴隨水熱處理的組別，細胞相較於其他表面處理接受到較大的應力刺激。

關鍵字

陽極氧化 ； 有限元素分析 ； 應力

並列摘要

In vitro cell response is believed related to the physical and chemical properties of substrate. In this study, the cell adhesion affected by mechanical stimulation from substrate was evaluated by culturing the MG-63 osteoblast-like cells on Ti plates with different chemical composition and surface topography. Three types of surface, surface with machined grooves, with pores, and with pillars, was fabricated by mechanically abraded (control), anodized (AO) and anodized following with hydrothermally treated (HYT) Ti plates, individually. Cells exhibited earlier spreading on the AO and HYT surface after 5 hours culturing, resulted from chemical factor, i.e., calcium and phosphate containing on the surface. After 24 hours cells completely flattened on the HYT surface but not on the AO surface; this improved cell adhesion behavior was primarily attributed to physical factor that is specific surface topography provides cell relatively large mechanical stimulation. The finite element method was used to evaluate the stress distributions which cells were sufferentrol group (P > 0.01); therefore it can explain the fact that the superior cell adhesion resud. For the HYT group, analyzed data indicated that cell received larger stress stimulation than colted from the specific geometry of HYT coated-surface.

並列關鍵字

anodization ； finite element analysis ； stress

參考文獻

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