不同鈦金屬表面狀況對於細胞體外貼附情形之探討

Cell adhesion on cp-titanium with different surface treatment

鄭証溢(Cheng-Yi Cheng)

指導教授 ： 陳文正

共同指導教授 ： 洪純正(Hung Chun CHeng)

高雄醫學大學/口腔醫學院/口腔衛生科學研究所碩士在職專班/碩士(2009年)

摘要

本研究是要探討利用不同表面處理方式對於鈦金屬表面進行處理，藉由各細胞的貼附情形，來探討適合各細胞生長貼附之表面狀況，利用不同的表面處理來平衡調整各細胞的生長速度，讓植體植入後的成功率能夠更為增加。研究所用的材料為商業用純鈦，所用的細胞分別為:骨母細胞(MC3T3-E)、纖維細胞(NIH 3T3)及表皮細胞(XB-2)，所使用的表面處理為不同時間的噴砂及不同時間的酸蝕，且利用四鈣磷酸鹽二次噴砂進行清潔表面及期望能提供骨母細胞生化所需之鈣離子，實驗結果顯示表皮細胞在噴砂60s酸蝕600s(Ra=0.74±0.13μm)初期貼附情形較好，而增生狀況則是噴砂30s酸蝕600s(Ra=0.73±0.12μm)；纖維細胞初期貼附是噴砂30s酸蝕600s(Ra=0.73±0.12μm)較好，而增生也是噴砂30s酸蝕600s(Ra=0.73±0.12μm)較好；骨母細胞初期貼附是在噴砂10s酸蝕600s(Ra=0.64±0.13μm)較好，而增生則是在噴砂60s酸蝕60s(Ra=1.02±0.08μm)較好。而從也結果發現噴砂10s酸蝕30s(Ra=0.67±0.07μm)的處理方式骨母細胞貼附較好，而纖維細胞及表皮細胞貼附較不好，再經四鈣磷酸鹽噴砂後，骨母細胞貼附情況反而下降，而四鈣磷酸鹽類萃取液對於細胞貼附的結果卻是有助於骨母細胞的貼附，由此可知對於細胞初期貼附而言型態效應是遠大於離子效應的。

關鍵字

鈦 ； 表面狀況 ； 細胞貼附

並列摘要

The purpose of this study is to explore whether using different kinds of surface treatment processes on titanium-made dental implants would affect the success rate of dental implantation. Investigations were performed by observing the growth and attachment conditions of different sorts of cell lines on the metal surface of titanium slices with different surface treatments. The metal material employed in this study was the commercially-used pure titanium, cell lines used were bone cells (MC3T3-E), fibroblasts(NIH 3T3) and epidermal cells (XB-2). Titanium slices were processed through sand-blasting at different time points and etching with different time points. In addition, we had utilized tetra-calcium phosphate to perform secondary sand-blasting process to clean up the metal surface and hope to provide the necessary calcium ions for osteoblastoma physiology.
Results showed that epidermal cells with 60s sand-blasting/600s acidic-etching (Ra=0.74 ± 0.13μm) had better attachment activity at initial stage and their proliferation status was better with 30s blasting/600s acidic-etching (Ra=0.73 ± 0.12μm); fibroblasts with 30s sand-blasting/600s acidic-etching (Ra=0.73 ± 0.12μm) had better attachment activity at initial stage and their proliferation status was better with 30s sand-blasting/600s acidic-etching (Ra=0.73 ± 0.12μm); Osteoblastoma cells with 10s sand-blasting/600s acidic-etching (Ra=0.64 ± 0.13μm) had better attachment activity at initial stage and their proliferation status was better with 60s blasting/60s acidic-etching (Ra=1.02 ± 0.08μm).
Results also showed that after 10s sand-blasting/ 30s acidic-etching (Ra=0.67 ± 0.07μm), osteoblastoma cells had better attachment activity. However, fibroblasts and epidermal cells did not attach as well as the osteoblastoma cells under the same conditions. It was worthwhile noticing that after sand-blasting with tetra-calcium phosphate, osteoblastoma cells had demonstrated reduced attachment activity. On the other hand, sand-blasting with the extracts of tetra-calcium phosphate had helped the osteoblastoma cells to attach better. Therefore, the morphological effect at initial stage of cell attachment should be greater than the ionic effect.

並列關鍵字

cp-titanium ； surface treatment ； Cell adhesion

參考文獻

1.鐘國雄(編著). 牙科材料學. 合記出版社,2004(第六刷)。

2. Steinemann S. Titanium-the material of choice? Periodontology 2000 1998;17:7–21.

3.Buser D, Broggini N, Wieland M, Schenk RK, Denzer AJ, Cochran DL,et al. Enhanced bone apposition to a chemically modified SLA titanium surface. J Dent Res 2004;83:529–33.

4. Zhao G, Schwartz Z, Wieland M, Rupp F, Geis-Gerstorfer J,Cochran DL, et al. High surface energy enhances cell response to titanium substrate microstructure. J Biomed Mater Res A 2005;74:49–58.

5. Bagno A, Di Bello C. Surface treatments and roughness properties of Ti-based biomaterials. J Mater Sci Mater Med 2004;15:935–49.