鈦金屬植體表面改質之細胞反應特性比較

Research of Various surface Functionalization on Titanium Implant invitro

后淑娟(Su-Jiun Hou)

指導教授 ： 歐耿良

臺北醫學大學/口腔醫學院/醫療器材產業碩士專班/碩士(2014年)

摘要

無資料

關鍵字

DLC膜

並列摘要

To evaluate the cell responses of titanium oxide layer with the nanoporous micro-structure, an electrochemical anodization approach was proposed to create a nanoporous structure that superimposed onto the moderately rough sand-blasted, large-grit, acid-etched (SLA) Ti surface (denoted as SLAffinity-Ti). The osteoblast-like cells were cultured on SLAffinity-Ti and un-treated surfaces. Cell adhesion, viability,mRNA expressions as well as the productions of alkaline phosphatase (ALP) and osteocalcin (OC) were evaluated at predetermined intervals. The present results showed that cells had a high viability, attachment, and improved cell proliferation at day 7 and 14 when cultured on SLAffinity-Ti surface. The mRNA expressions of ALP and OC were upregulated while the type I collagen was not influenced. Additionally, the cells cultured on the SLAffinity-Ti surface produced a higher level of ALP and OC relative to cells on untreated surfaces. These findings confirmed that surface topography (nanoporous roughness) and TiO2 layers influenced the cell responses and the study revealed that cells grown on SLAffinity-Ti may improve osseointegration for Ti implants.

並列關鍵字

DLC film ； titanium dopant ； biocompatibility and densification

參考文獻

1. J. Lincks, B. D. Boyan, C. R. Blanchard, C. H. Lohmann, Y. Liu, D. L. Cochran, D. D. Dean, and Z. Schwartz, Biomaterials, 19(23), 2219 (1998).

2. M. Long and H. J. Rack, Biomaterials, 19(18), 1621 (1998).

3. A. Wennerberg and T. Albrektsson, Clin Oral Impl Res, 20(Suppl 4), 172 (2009).

4. D. Buser, N. Broggini, M. Wieland, R. K. Schenk, A. J. Denzer, D. L. Cochran, B. Hoffmann, A. Lussi, and S. G. Steinemann, J Dent Res, 83(7), 529 (2004).

5. H. Ikeda, P. E. Rossouw, P. M. Campbell, E. Kontogirogos, and P. H. Buschang, Am J Orthod Dentofacial Orthop, 139(2), e153 (2011).