飲食中給予不同總量與型態的脂肪酸對倉鼠體重、體脂蓄積及血脂代謝之影響

Effects of different amounts and types of dietary fatty acids on body weight, fat accumulation and lipid metabolism in hamsters

楊濟華(Ji-Hua Yang)

指導教授 ： 簡怡雯

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2013年)

摘要

本篇研究目的為探討膳食油脂中不同總量與型態之脂肪酸對倉鼠體重、體脂蓄積及血脂代謝之影響。實驗採用60隻7週齡雄性倉鼠，飲食以AIN-93M為主，經過一週的適應期後，隨機分成6組，分別給予5% (w/w)、15%及20%大豆油，簡稱為S5、S15及S20組和5%、15%及20%實驗調和油，簡稱為M5、M15及M20組，調和油組成為含60%單元不飽和脂肪酸，而多元不飽和與飽和脂肪酸比值為5(MUFAs 60%, P/S ratio 5)，實驗期間自由攝取飲食和水，每週固定測量體重和攝食情形，12週後予以犧牲。結果顯示S20組的體重和體重增加量顯著大於其他五組，主要增加脾臟、腎臟、副睪及腹膜後白色脂肪組織重；在S組間，當油脂攝取量增加，體重、體重增加量及脂肪重會顯著上升，而M組則無顯著差異。M15血清低密度膽固醇濃度顯著低於S15組；S20與M20組相比，顯示S20組有顯著較高的瘦體素、胰島素濃度及促三酸甘油酯累積之酵素lipoprotein lipase(LPL)，而促脂肪分解酵素acetyl-CoA oxidase (ACO)和carnitine palmitoyltransferase-1 (CPT-1)則顯著較低。因此，在高油飲食下，特殊比例脂肪酸的調和油會藉由顯著降低胰島素濃度、LPL酵素活性及顯著增加肝臟脂質分解酵素活性來防止體重和體脂蓄積。

關鍵字

關鍵詞：總脂肪攝取量 ； 飽和脂肪酸 ； 單元不飽和脂肪酸 ； 多元不飽和脂肪酸 ； 體脂蓄積

並列摘要

In this study, we explore the effects of different amounts and dietary fatty acids on body weight, fat accumulation and lipid metabolism in hamsters. Sixty male golden Syrian hamsters were randomly divided into six groups. Three of the groups fed with experimental diets containing 5%, 15% and 20% (w/w) fat of soybean oil (S5, S15 and S20). And the other three groups fed with experimental mix oil (M5, M15 and M20). According to the AIN-93M formulation and modification for 12 wk, experimental mix oil composition consist of 60% monounsaturated fatty acids (MUFAs) and polyunsaturated-to-saturated fatty acid (P/S) ratio 5 mix by soybean oil and canola oil. All animals were given ad libitum access to food and water. Consumption of food was daily measured, and body weight was weekly measured. The Result showed that the S20 group’s weight and weight gain were significantly greater than the other five groups. The weight gain was main increasing in spleen, kidney, epididymal and retroperitoneal white adipose tissue. When the total fat consumption increased, the S group’s body weight, weight gain and adipose tissue weight will significantly increase, but they were no significant difference in the M groups. The serum low-density lipoprotein cholesterol concentrations were significantly lower between M15 group and S15 group. When S20 compared with M20 group, S20 had significantly higher leptin, insulin concentration and promote triglyceride accumulation enzyme lipoprotein lipase (LPL), but lipolysis enzyme acetyl-CoA oxidase (ACO) and carnitine palmitoyltransferase-1 (CPT-1) was significantly lower. Therefore, the study showed that special proportion of experimental mix oil (MUFAs 60%, P/S ratio 5) with high fat can prevent body weight gain and body fat accumulation by lowering insulin concentration and increased hepatic lipolytic enzyme activities.

並列關鍵字

Keywords: total fat consumption ； saturated fatty acids ； monounsaturated fatty acids ； polyunsaturated fatty acids ； body fat accumulation.

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