探討SCRN1(Secernin 1)在乳癌中細胞增生所扮演之角色

Studies on the Role of SCRN1 (Secernin 1) in proliferation of breast cancer cells

黃郁婷(Yu-Ting Huang)

指導教授 ： 何元順

臺北醫學大學/醫學科技學院/醫學檢驗暨生物技術學系/碩士(2011年)

摘要

本篇研究主要是要探討SCRN1在乳癌細胞中所扮演的角色。根據之前的文獻發現SCRN1在乳癌細胞中表現量比正常的乳房細胞來的高，文獻利用microarray的方法分析幾株乳癌細胞株中基因的表現量(Charafe-Jauffret, Ginestier et al. 2006)，但並沒有進一步探討基因跟癌症發生的可能性為何。
在本篇論文中主要想要研究的是SCRN1在乳癌中所扮演的角色，首先利用了乳癌病人的檢體，經由Real-time PCR分析，發現SCRN1在腫瘤表現相對高於腫瘤周邊的組織，而在組織切片染色(IHC)，可以在顯微鏡下觀察到SCRN1在腫瘤細胞的表現多於正常乳房上皮細胞的表現。接下來我們也在細胞實驗中想要探討SCRN1在細胞中的角色對於腫瘤是不是有直接的相關性，我們利用了siRNA送入細胞，顯示SCRN1被knock down後細胞的生長速率降低。乳癌細胞中的SCRN1的表現會被Nicotine所誘導，且發現天然藥物Inotilone和EGCG會抑制SCRN1的表現。並發現細胞過度表現SCRN1對於細胞生長有影響。

關鍵字

乳癌

並列摘要

In this study we focus on the correlation between SCRN1 and breast cancer.The previous studies find out the expresstion of SCRN1 in breast cancer cell is truly higher than the noromal breast epithelial cell.The data use microarray to screen a few cell lines to find out how they express,but did not show gene’s function in the cancer cells.
In this study we want to research for the function of SCRN1 in breast cancer cell.First,we used Real-time PCR to analyze the expression of SCRN1 in the clinical patient tissues.We find out SCRN1 in breast cancer cell expression is higher than noromal breast epithelial cells ,so is the IHC data. Furthermore,we want to find out what is the relationship with SCRN1 in the breast cancer cell.We use RNAi to knock down SCRN1 in breast cancer cells,shows that cell growth can be suppress. On the other hand, we use transfection to overexpress SCRN1 to see if the cell growth will change.

並列關鍵字

SCRN1

參考文獻

Charafe-Jauffret, E., C. Ginestier, et al. (2006). "Gene expression profiling of breast cell lines identifies potential new basal markers." Oncogene 25(15): 2273-84.

Chen, C. S., C. H. Lee, et al. "Nicotine-induced human breast cancer cell proliferation attenuated by garcinol through down-regulation of the nicotinic receptor and cyclin D3 proteins." Breast Cancer Res Treat.

Hartwell, L. H. and M. B. Kastan (1994). "Cell cycle control and cancer." Science 266(5192): 1821-8.

Kuo, Y. C., C. S. Lai, et al. (2009). "Differential inhibitory effects of inotilone on inflammatory mediators, inducible nitric oxide synthase and cyclooxygenase-2, in LPS-stimulated murine macrophage." Mol Nutr Food Res 53(11): 1386-95.

Massague, J. (2004). "G1 cell-cycle control and cancer." Nature 432(7015): 298-306.