台灣人種之IL-17A、CD14、MMP-1和TLR4單一核苷酸多型性與侵犯性牙周炎之間關聯性

本研究目的在探討台灣人種單一核苷酸的基因多型性與侵犯性周炎有無顯著的關聯性，研究對象人種均為台灣人，實驗方式採取病例對照研究設計(case-control study)，將其分為侵犯性牙周炎、慢性牙周炎與牙周健康三組，選取的四種單核苷酸分別座落於白介素17A (interleukin-17A，簡稱IL-17A)、表面抗原分化簇14 (cluster of differentiation 14，簡稱CD14)、間質金屬蛋白酵素1 (matrix metalloproteinase-1，簡稱MMP-1)與類鐸受體4 (toll-like receptor 4，簡稱TLR4)基因上，從所獲得的血液樣本，利用即時聚合酶鏈式反應(Real-time polymerase chain reaction，簡稱Real-time PCR)，藉由PCR擴增的原理，使極微量的DNA 放大，再經由螢光訊號偵側系統達到即時偵測(Real-time)的效果，來定量此四種基因多型性的表現。基因型及對偶基因之頻率(genotype and allelic frequencies)於侵犯性牙周炎和慢性牙周炎(aggressive periodontitis and chronic periodontitis)與牙周健康相比較時，將採用卡方檢定(Chi-square tests)或費雪精確檢定(Fisher’s exact test)，而邏輯迴歸(logistic regression)分析用來測量基因型與牙周炎之間關聯性。結果顯示牙周炎(侵犯性牙周炎與慢性牙周炎)的患者有較高表面抗原分化簇14的對偶基因G (侵犯性牙周炎:39.3%，慢性牙周炎:38%，牙周健康:6.8%)；侵犯性牙周炎有較高間質金屬蛋白酵素1的基因型1G/1G與對偶基因1G (侵犯性牙周炎:66.1%，慢性牙周炎:40%，牙周健康:34.1%)；至於白介素17A與類鐸受體4，與牙周炎並無關聯性。合併與侵犯性牙周炎有關的單一核甘酸多型性(表面抗原分化簇14與間質金屬蛋白酵素1)的對偶基因有較高的百分比罹患侵犯性牙周炎。本實驗結果暗示表面抗原分化簇14基因多型性(對偶基因G)跟台灣人種罹患侵犯性牙周炎與慢性牙周炎有關，而間質金屬蛋白酵素1基因多型性(對偶基因1G)可能跟侵犯性牙周炎有關，此外合併帶有跟侵犯性牙周炎有關的對偶基因，有可能會提高侵犯性牙周炎的感受性。

關鍵字

基因多型性；侵犯性牙周炎；慢性牙周炎

並列摘要

The aim of the present study was to investigate the association of single nucleotide polymorphism (SNP) with aggressive periodontitis (AgP) in Taiwanese. All subjects were Taiwanese ethnicity. The design of study was a case-control study, including the test groups of aggressive periodontitis (AgP) and chronic periodontitis (CP) volunteers, and the control group of periodontitis-free healthy participants. SNPs of interleukin-17A (IL-17A), cluster of differentiation (CD14), matrix metalloproteinase-1 (MMP-1) and toll-like receptor 4 (TLR4) were chosen. According to the principle of polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR) was utilized to amplify the small quantity of DNA from the peripheral blood sample. The effect of Real-time was achieved to determine the expression of the four SNPs quantitatively by using ﬂuorescence signal detection system. We used the chi-square test or Fisher’s exact test to compare the means and proportions in allele/genotype frequencies of the three groups. Logistic regression analysis was performed to measure the relationship between the genotypes and periodontitis. Analysis of the polymorphisms showed patients with periodontitis (AgP and CP) had a higher distribution of G allele (AgP:39.3%, CP:38%, H:6.8%) for CD14 SNPs, patients with AgP had higher 1G/1G genotype and 1G allele (AgP:66.1%, CP:40%, H:34.1%) for MMP-1 SNPs (p<0.05). As for IL-17A and TLR4 SNPs, we found no correlation with periodontitis. The subjects with the combined allele associated with AgP had a higher percentage than the subjects with the single allele in AgP group. These data suggest that patients with CD14 polymorphism were associated with the occurrence of AgP and CP in Taiwanese ethnicity population. The MMP-1 polymorphism (1G allele) is genetically associated with vulnerability of patients with AgP. In addition, combined occurrence of the CD14 and MMP-1 SNPs allele may raise the susceptibility of one individual to AgP.

並列關鍵字

single nucleotide polymorphism (SNP) ； aggressive periodontitis ； chronic periodontitis

參考文獻

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台灣人種之IL-17A、CD14、MMP-1和TLR4單一核苷酸多型性與侵犯性牙周炎之間關聯性

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