松杉靈芝之補充對大鼠之運動表現及力竭運動後抗氧化狀態之影響

Effects of Ganoderma tsugae supplementation on exercise performance and antioxidant status after exhaustive exercise in rats

詹芷琪(Chih-Chi Chan)

指導教授 ： 楊素卿

臺北醫學大學/公共衛生暨營養學院/保健營養學研究所/碩士(2007年)

https://doi.org/10.6831/TMU.2007.00115

摘要

本研究之目的為探討松杉靈芝之補充對大白鼠因力竭運動所導致的抗氧化狀態不平衡及其運動表現之影響。利用三十隻雄性Sprague-Dawley大白鼠，經一週適應期後，跑步機適應較佳的二十四隻，以隨機方式分為力竭運動（E）、力竭運動補充低劑量（0.1875 g/kg BW）（EL）、中劑量（0.9375 g/kg BW）（EM）及高劑量（1.875 g/kg BW）松杉靈芝（EH）等四組，其餘六隻則為控制組（C）。經三十天餵養後，控制組大白鼠立即犧牲，運動之四組大白鼠以跑步機經力竭運動後予以犧牲，採集其血液、肌肉及肝臟樣本。結果發現，能量代謝方面，與C組相較，E組之肌肉肝醣含量、血糖濃度顯著降低、血尿素氮濃度顯著增加（p < 0.05）。而與E組相較之下，EL組血中游離脂肪酸濃度顯著降低，EM組血糖濃度及肝醣含量顯著降低，而EH組肝醣含量顯著降低（p < 0.05），其餘數據皆無顯著差異。跑步時間方面，EL組、EH組較E組顯著增加（p < 0.05），且EH組又比EL組顯著增加（p < 0.05），而EM組只有增加之趨勢。抗氧化狀態方面，E組之紅血球SOD（superoxide dismutase）、CAT（catalase）、GRD（glutathione reductase）活性及GSH/GSSG比值與C組相較顯著降低（p < 0.05），而EL、EM及EH組其SOD、CAT、GPX（glutathione peroxidase）、GRD活性及GSH/GSSG與E組則無顯著差異，但EH組血漿TBARS濃度則顯著低於E組（p < 0.05）。組織損傷方面，E組血漿中AST（aspartate transaminase）、ALT（alanine transaminase）、CK（creatine kinase）及LDH（lactate dehydrogenase）活性顯著高於C組（p < 0.05），肌肉細胞凋亡情形也較嚴重。與E組相較，EL、EM及EH組其AST、ALT、CK及LDH活性皆無顯著差異，肌肉細胞凋亡情形則有較低的現象。以上結果顯示，大白鼠力竭運動前補充低劑量（0.1875 g /kg BW）松杉靈芝，可維持力竭運動後其血糖恆定及肌肉肝醣含量，並減少肌肉細胞凋亡的情形，而補充高劑量（1.875 g /kg BW）松杉靈芝，除上述作用外，更可增進脂肪利用及減少脂質過氧化物產生，以達延長運動時間之效果。

關鍵字

松杉靈芝 ； 力竭運動 ； 運動表現 ； 細胞凋亡

並列摘要

The purpose of this study was to investigate the effects of Ganoderma tsugae (GT) supplementation on exercise-induced imbalance of antioxidative status and exercise performance in rats. Thirty male Sprague-Dawley rats were divided into five groups: control (C), exhaustive exercise (E), exhaustive exercise with low dose (0.1875 g/kg BW) (EL), middle dose (0.9375 g/kg BW) (EM) and high dose (1.875 g/kg BW) GT supplementation (EH) groups. After 30 days, the rats of exercise groups underwent an acute exhaustive running test on a motorized treadmill. After the running test, all rats were sacrificed immediately. The blood, muscle and liver samples were collected. The energy metabolism, the muscle glycogen content and blood glucose were significantly decreased and blood urea nitrogen (BUN) was increased in E group (p<0.05), compared with C group. Compared with E group, the free fatty acids (FFA) concentration in EL group, blood glucose concentration in EM group, and the liver glycogen content in EM and EH group were significantly decreased (p<0.05). On the other hand, the running time was obviously prolonged in EL and EH group than that of E group (p<0.05), and it significantly in EH group than that of EL group (p<0.05). For the antioxidative status, the activities of superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRD) and GSH/GSSG ratio in the erythrocytes were obviously reduced (p<0.05). There were no significant differences in SOD, CAT, glutathione peroxidase, GRD activities, and GSH/GSSG ratio in EL, EM and EH group, when compared with E group. TBARS level of EH group was apparently decreased than that of E group (p<0.05). About the tissue damage, the activities of aspartate transaminase (AST), alanine transaminase (ALT), creatine kinase (CK) and lactate dehydrogenase (LDH) in the plasma were significantly raised in E group (p<0.05), when compared with C group (p<0.05). The apoptosis of muscle cell in E group was more apparent than in C group. The plasma AST, ALT, CK and LDH activities in EL, EM, and EH group had no changes, when compared with E group. The apoptosis of muscle cell in EL, EM, and EH group was less apparent than in E group. The results show that low dosage supplementation of Ganoderma tsugae before exhaustive exercise may maintain glucose homeostasis, muscle glycogen content and attenuate apoptosis of muscle cell to prolong running time.

並列關鍵字

Ganoderma tsugae ； exhaustive exercise ； exercise performance ； apoptosis

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