奈米自組裝仿生結構於氧化鈦之研究

Research of Biomimetic Nanostructure on Titanium Oxide by Self-Assembly

趙剛(Kang Chao)

指導教授 ： 歐耿良

臺北醫學大學/口腔醫學院/生醫材料暨組織工程研究所/碩士(2013年)

摘要

本研究之目的是藉由自組裝技術於氧化鈦薄膜上建立具奈米仿生結構之表面，利用長鏈烷基磷酸酯溶液於氧化鈦薄膜上建立具生物活性與低楊氏模數之自組裝單層膜。本實驗研究探討此自組裝單層膜之表面形貌、表面粗糙度、表面潤濕性、化學成分、機械性質與生物相容性。實驗結果顯示氧化鈦薄膜經自組裝處理後其平均粗糙度及方均根粗糙度皆有下降之趨勢，且自組裝單層膜之表面粗糙度隨者碳鏈的增加而漸增；接觸角實驗中發現羧基修飾之自組裝單層膜的與去離子水及PBS之接觸角分別為73.4∘、68.85∘；此外經連續剛性測量模式(QCSM)奈米壓痕研究指出經自組裝修飾之氧化鈦表面具有較低之接觸剛度；最後從細胞體外實驗結果顯示，氧化鈦薄膜經烷基磷酸修飾後之表面對於骨肉瘤細胞有較好之附著性。綜合上述，氧化鈦薄膜經長鏈烷基磷酸酯進行自組裝反應後可有效降低其接觸剛度、並提高材料表面之生物相容性，未來期望可應用於生醫植入物之表面改質。

關鍵字

氧化鈦 ； 自組裝單層膜 ； 奈米壓痕 ； 接觸剛性

並列摘要

The purpose of this study is to fabricate a biomimetic nanostructure on titanium oxide film by self-assembly technique. Bioactive film with low elastic modulus formed on titanium oxide surface was fabricated by self-assembled monolayers (SAMs) in alkyl phosphate solutions. The surface morphology, wettability, roughness, chemical composition, stiffness, and biocompatibility of the SMAs-treated layer were investigated. The SAMs-treated coatings with roughness in nanometer scale were more hydrophilic than bare titanium oxide. Furthermore, the elastic moduli of the SAMs-treated surface, examined by nanoindentor with quasi continuous stiffness method (QCSM) testing, were lower than that titanium oxide. The hydrophilic surface with low stiffness is attributed to the SAMs-treated coating covalently bonded to titanium oxide. Finally, in vitro test demonstrated that the SAMs-treated surface was favourable for MG63 osteoblast-like cells attachment. In conclusion, titanium oxide surface with self-assembled monolayers by alkyl phosphate can induce the contact stiffness, and increase the biocompatibility of titanium oxide. Expectations of future, the self-assembled technology can be applied on the surface treatment of biomedical implants.

並列關鍵字

Titanium oxide ； Self-assembled monolayers ； Nanoindentation: Contact stiffness

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