荔枝種子之成分及抑制黑色素生成之研究

Chemical Constituents and Melanogenesis-Inhibitory Activities of Litchi chinensis seeds

許雅萍(Ya-Ping Hsu)

指導教授 ： 李慶國

臺北醫學大學/藥學院/生藥學研究所/碩士(2013年)

摘要

荔枝，為無患子科的植物，生存在亞熱帶、中國南部和台灣等地區。荔枝的果皮呈暗紅色，有瘤狀突起，乾且薄；果肉呈白色透明狀，厚而多汁。根據文獻，荔枝籽具有良好的抗氧化活性；而具有抗氧化活性可能也會具有降低色素沉澱的效果。
將荔枝籽陰乾後，以95%的酒精萃取三次，濃縮後得598.4克。再經過分配萃取得到乙酸乙酯層98.1克、正丁醇層198.5克和水層210.8克。而經由B16-F10黑色素細胞和酪胺酸酶酵素的活性篩選，乙酸乙酯層皆有最好的效果。取乙酸乙酯層進行管柱層析。經過NMR、MS的鑑定後，共得到20個化合物。
20個化合物為10個phenolic類化合物、2個coumarin類化合物
、4個fatty acid類化合物、1個flavonoid類化合物及3個其他類化合物。其中化合物LCS8 (3,4-dihydroxybenzoic acid ethyl ester) 在抑制酪胺酸酶酵素有最好的效果。但在黑色素細胞實驗上具有細胞毒殺性。

關鍵字

荔枝 ； 黑色素

並列摘要

Litchi chinensis, belonging to family Sapindaceae, is a king of subtropical fruit tree and famous for long time in Southeast Asia and Taiwan. The fruits of Litchi chinensis are called “ Lychee ” has dry, red pericarp and white edible aril. The seeds extract of lychee may reduce hyperpigmentation effect. In this study, the seeds of lychee was extracted with 95% ethanol and sequentially partitioned with ethyl acetate, n-butanol and water. Therefor, the ethyl acetate fraction with the higher inhibitory activity for tyrosinase and reduced hyperpigmentation for B16-F10 melanoma cells. The fraction was subjected to silica gel column chromatography. Twenty compounds were obtained and isolated. The structures were identified by NMR, MS and literature data. LCS 8 was inhibit tyrosinase and prevent melanin production. But it has cytotoxicity, how to reduce its toxicity with other ingredients will be the future research work.

並列關鍵字

Litchi ； melanin

參考文獻

參考文獻 (References)

1. Asta Juzeniene, R.S., Alina Porojnicu, Arnfinn Hykkerud Steindal, Johan Moan, Development of different human skin colors: A review highlighting

photobiological and photobiophysical aspects. Journal of Photochemistry and Photobiology B: Biology, 2009. 96: p. 93–100.

2. Aoki, K., Sexual selection as a cause of human skin colour variation: Darwin's hypothesis revisited. ANNALS OF HUMAN BIOLOGY, 2002. 29(6): p. 589 - 608.

3. Kristin K. Deeb, D.L.T.a.C.S.J., Vitamin D signalling pathways in cancer: potential for anticancer therapeutics. Nature Publishing Group, 2007. 7.