血小板濃厚液於骨質疏鬆之骨再生研究：轉換及平衡骨新生與脂肪新生作用

The balance between adipogenesis and osteogenesis in bone regeneration by platelet-rich plasma for age-related osteoporosis

劉恆宇(Hen-Yu Liu)

指導教授 ： 蘇慶華

共同指導教授 ： 鄧文炳

臺北醫學大學/醫學院/醫學科學研究所/博士(2011年)

https://doi.org/10.6831/TMU.2011.00157

摘要

骨質疏鬆症（osteoporosis）為體內骨質大量流失所造成之疾病，預防該疾病之方法為促進骨再生作用，以達治療骨質疏鬆之效果。本實驗室於先前已證實PRP具誘發骨前驅細胞（osteo-progenitor cell）增生及骨分化（osteogenesis)之能力，進而達到治療骨質疏鬆症之效果；因此本論文更證實PRP不僅可促進骨生成作用，同時具抑制脂肪新生作用（adipogenesis）之能力。鑑此本實驗所發展之PRP促進骨生成平台，結果證實PRP不僅可促進骨分化，亦可改善骨質疏鬆症老鼠之骨鬆現象，且更進一步印證PRP具抑制脂肪細胞分化及誘導脂肪細胞轉分化（transdifferentiation）為類骨細胞之能力；於動物實驗方面，以1個月及10個月年齡之早老化老鼠，模擬骨鬆前後之動物疾病模式，結果發現PRP不僅可促進早老化老鼠骨髓內之骨新生作用，亦可抑制脂肪新生作用，進而達到預防(年輕)及治療(老年)骨鬆老鼠之效果。本研究所發展PRP之骨生成平台，預期能作為後續以骨鬆研究為基礎之臨床發展。

關鍵字

骨質疏鬆，生長因子複合物，骨鬆老鼠，脂肪新生，預防和治療

並列摘要

The major cause of senile osteoporosis is the loss of functional osteoblasts which are the primary target in formulating a more effective therapy. Our previous studies have demonstrated that NIH3T3 embryonic fibroblasts were predifferentiated into osteoblast-like cells using platelet-rich plasma (PRP) medium, and transplanted into ovariectomized senescence-accelerated mouse prone substrain 8 (OVX-SAMP8 mice) to prevent osteoporosis and prolong the lifespan of animals. In this study, we further demonstrated that PRP treatment mainly exerted its action via promoting bone regeneration but also appeared to suppress adipogenesis within the marrow. These results developed two major issues in this thesis. First we developed that the administration of PRP not only induced osteoblast differentiation but also successfully reversed osteoporosis in osteoporotic mice. Therefore, we established the pre-adipocyte cell model of adipogenesis to study whether the PRP could regulate the adipocyte differentiation and transdifferentiate to the osteoblast like cell. In in vivo study, Young (for prevention) and Old (for treatment) OVX-SAMP8 mice were used the osteoporotic animal disease model. These animals studies demonstrated that PRP could not only increase bone regeneration but also reduced bone marrow adiposity in the bone environment. These findings suggest that PRP could potentially be applicable for prevention and treatment of senile postmenopausal osteoporosis in pre-clinical.

並列關鍵字

osteoporosis, platelet-rich plasma (PRP), ovariectomized senescence accelerated mice (OVX-SAMP8), adipocyte, prevention and treatment

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