幾丁聚醣膜材表面微構形對PC12細胞生長行為表現之研究

The Study of the Behaviors of PC12 Cells on the Micropatterned Chitosan Membrane

周佩洵(Chou Pei Hsun)

指導教授 ： 孫一明

元智大學/工程學院/化學工程與材料科學學系/碩士(2004年)

摘要

本研究以軟蝕刻與微接觸拓印的技術，於幾丁聚醣膜材表面形成具有立體幾何與生物化學特性差異的直線微構形，探討立體結構以及層黏連蛋白（laminin）對 PC12細胞分佈情形，以及構形寬度對導引細胞分化之神經纖維生長方向之影響。實驗結果發現，PC12細胞於線寬/線距為200 μm以下之立體微構形，侷限於構形凹處；而不論線寬/線距大小，PC12細胞優先選擇排列於之laminin微構形上。線寬/線距為20/20μm 的立體與蛋白質微構形，對神經纖維生長方向皆有最佳的導引效果。
此外，藉由分析幾丁聚醣膜材降解三個月後，重量損失、機械強度與熱性質之分析，探討材料隨時間降解之性質變化，比較材料於擬生理緩衝溶液中與溶菌酶作用下，降解速率之差異。由重量損失、拉力實驗與TGA測試實驗結果發現，幾丁聚醣膜材於溶菌酶作用下，雖可加速其降解反應，但降解程度仍與預期差距甚大，應受限於幾丁聚醣之高去乙醯化程度所致。

關鍵字

軟蝕刻技術 ； 微接觸拓印 ； 微構形 ； 幾丁聚醣 ； 生物可降解性 ； PC12細胞

並列摘要

In this study, both topological and protein micropatterned surfaces were designed and generated on chitosan membranes by using soft lithography and micro-contact printing techniques. The distribution and the morphology of PC12 cells and the orientation of the neuritis were observed on the patterned surface of various spacing size. PC12 cells localized in the grooves with the pattern spacing size less than 200 μm on the topological micropatterned surface. On the contrary, PC12 cells selectively adhered on the protein region of any size on the laminin micropatterned surface. In addition, the neurites of PC12 cells exhibited the best orientation either on the topological or protein micropatterns with spacing size of 20 μm.
The degradation of chitosan membranes in PBS or lysozyme/PBS was characterized by monitoring the loss in weight as well as the mechanical and thermal decomposition properties for three months. The presence of lysozyme accelerated the degradation; however, the degradation rate was slower than expected because the degradation activity of lysozyme was limited by the high degree of deacetylation of chitosan.

並列關鍵字

Soft lithography ； micro-contact printing ； micropattern ； chitosan ； biodegradable ； PC12 cell

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