- 學位論文
利用全場外差干涉術探討葡萄糖氧化酵素對葡萄糖溶液濃度之二維動態分析
Dynamic Analysis of the Glucose Concentration Reacted With Glucose Oxidase by Using Full-Field Heterodyne Interferometry
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摘要
本研究應用全場外差干涉術在動態反應的量測上，並完成了葡萄糖感測試片在偵測葡萄糖濃度之二維反應趨勢。由於感測試片上佈植之葡萄糖氧化酵素(GOx)具有對葡萄糖的化學反應專一的特性，故可完全反映待測溶液中葡萄糖濃度，不受溶液中其他化學成分之干擾。此外，結合全場外差干涉相位量測技術，可獲得感測試片上GOx與葡萄糖反應之二維狀態，有利於GOx佈植於玻璃板上之特性分析。此外，本研究並探討GOx與待測溶液間pH值差異對量測反應時間及量測靈敏度的影響，以及GOx濃度對量測反應時間及量測靈敏度的關聯性。由實驗結果顯示，本研究之反應時間優於2.2秒且系統解析度可達39.286 mg/dL。
並列摘要
In this study, we demonstrated a full-field heterodyne interferometer to analyze two dimensional (2D) dynamic phase variation of the chemical reaction between the testing sample and the glucose sensor. Because of high selectivity of the glucose oxidase (GOx), we can ensure the phase variation coming from the reaction between GOx and glucose dissolved in the complicated testing sample. Based on the advantages of the full-field heterodyne interferometer, we can investigate the 2D reaction behavior of the GOx under different immobilized pH value and concentration. According to our results, the best response time is shorter than 2.2 seconds observed in this study. We also obtained the calibration curve with high linearity within the glucose concentration of 100 ~ 600 mg/dL. Finally, we can get the resolution of this method can be reached to 39.286 mg/dL.
參考文獻
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