光合微生物燃料電池開發之研究

Development of Phototrophic Microbial-based Fuel Cell

林揚盛(Yang-Sheng Lin)

指導教授 ： 藍祺偉

元智大學/工程學院/化學工程與材料科學學系/碩士(2009年)

https://doi.org/10.6838/YZU.2009.00324

摘要

本研究以光合細菌(Rhodopseudomonas palustris)為生物觸媒進行光合微生物燃料電池開發與研究，研究的項目包括(1)電極改質(modified electrode)之影響;(2)介電質(mediator)型態之探討及(3)光合微生物電池於黑暗下或不同光照強度進行化學異營或光合異營之電池效能比較。研究結果得知，改質電極方面，使用奈米碳管石墨板電極分析電池效能可得到最大功率密度為3.26 mW m-2，其中最大的功率輸出分別為粗糙化石墨板的7.4倍、石墨板的12.7倍、不銹鋼板的108倍。以介電質Neutral red (NR)固定於石墨板電極，並且利用固定化之電極配合懸浮NR於陽極中，有最大的6.29庫倫輸出表現。探討光照影響研究發現，以葡萄糖為碳源在黑暗下進行化學異營可以擁有最高開環電壓230 mV和最低內電阻10 kΩ，在光照下進行光合異營則具有平穩的電功率輸出優點，本研究可以發現光合微生物燃料電池具有輸出平穩功率和利用光合作用增加電池壽命之效果，為具有應用價值潛力之微生物燃料電池。

關鍵字

光合微生物燃料電池 ； 光合作用 ； 光照強度 ； 介電質 ； 電極改質

並列摘要

In this study, the Rhodopseudomonas palustris was employed as bio-catalyst for development of Photomicrobial fuel cell (PMFC). The research focus upon (i) impact of modified electrodes on performance of biofuel cell; (ii) comparison of biofuel cell efficiency by the application of mediators and (iii) determination of biofuel cell under photohetrophic and chemohetrophic circumstance. The results demonstrated that carbon nanotube graphite of electrode presents more surface area from cyclic voltammograms analysis and generate the highest power output as compared to those graphite and rough-graphite electrodes. Application of mediator of suspended or immobilized neutral red could improved the PMFC performance. The combination of both can yield maximum coulomb. During chemohetrophic determination, PMFC had high cell performance while for photohetrophic, PMFC had stable power output. The results obtained from this study concluded the PMFC could be a promising vehicle for the requirement of energy supply and sustainable development.

並列關鍵字

Phototrophic micro-based fuel cells ； photosynthesis ； light intensity ； Rhodopseudomonas palustris ； modified electrode ； mediator

參考文獻

Bullen, R. A., T. C. Arnot, J. B. Lakeman, and F. C. Walsh, 2006, Biofuel cells and their development: Biosensors and Bioelectronics, v. 21, no. 11, p. 2015-2045.

Chaudhuri, S. K., and D. R. Lovley, 2003, Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells: Nat Biotechnol., v. 21(10), p. 1229-1232.

Chiao, M., K. B. Lam, and L. Lin, 2006, Micromachined microbial and photosynthetic fuel cells: Journal of Micromechanics and Microengineering, v. 16, no. 12, p. 2547-2553.

Duine, J. A., 1999, The PQQ story.: J Biosci Bioeng., v. 88, p. 231-236.

Dumas, C., A. Mollica, D. ron, R. Basseguy, L. Etcheverry, and A. Bergel, 2008, Checking graphite and stainless anodes with an experimental model of marine microbial fuel cell: Bioresource Technology, v. 99, no. 18, p. 8887-8894.