台灣產天仙果莖部細胞毒及化學成分之研究

Cytotoxic and Chemical Constituents from the Stem of Formosan Ficus formosana f. formosana

許雅雯(Ya-Wen Hsu)

指導教授 ： 蔡 ? 力

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2003年)

摘要

為了探討臺灣產桑科植物天仙果(Ficus formosana Maxium. f. formosana)對人類肝癌、血癌和淋巴癌細胞株活性成分，先後對其莖部的正己烷可溶部和氯仿可溶部進行活性成分分離，共得到20個化合物，包括三個chromene類：alloptaeroxylin (1)、ficuformodiol A (2)、ficuformodiol B (3)；五個flavonoid類：carpachromene (4)、5-hydroxy-8,8-dimethy-2-phenyl-8H-pyrano[3,2-g]chromen-4-one (5)、apigenin (6)、steppogenin (7)、glabranin (8)；一個cinnamic acid類：chromenylacrylic acid (9)；一個isocoumarin類：(R)-(-)-mellein (10)；四個triterpenoid類：squalene (11)、taraxeryl acetate (12)、bauera-7,9(11)-dien-3b-yl acetate (13)、oleana-11:13(18)-dien-3b-yl acetate (14)；六個steroid類：b-sitosterol (15) 和stigmasterol (16) 的混合物、 b-sitostenone (17)和stigmasta-4,22-dien-3-one (18) 的混合物、b-sitosterol-3-O-b-D-glucoside (19) 和b-stigmasterol-3-O-b-D- glucoside (20)的混合物。這些化合物均由光譜分析來決定其構造式。
其中，化合物2及3為天然首次分離之新化合物。將分離的化合物對人類四種癌細胞株包括未帶有B型肝炎病毒[HBV Ag (-)]的人類肝癌細胞株(HepG2)、帶有B型肝炎病毒[HBV Ag (+)]的人類肝癌細胞株(PLC/PRF/5)、血癌(THP-1)和淋巴癌(Raji)細胞株進行細胞毒體外活性試驗，結果發現化合物4和6對人類肝癌細胞株(HepG2和PLC/PRF/5)和淋巴癌細胞株(Raji)具有毒殺作用。

關鍵字

天仙果

並列摘要

The cytotoxic constituents against hepatoma, leukemia and lymphoma cancer cell lines in vitro were performed the studies on the stems of Ficus formosana Maxium. f. formosana (Moraceae). Investigation of the n-hexane- and CHCl3-soluble layers led to the isolation of 20 compounds, including three chromenes: alloptaeroxylin (1), ficuformodiol A (2), ficuformodiol B (3); five flavonoids: carpachromene (4), 5-hydroxy-8,8-dimethy-2-phenyl-8H-pyrano- [3,2-g]chromen-4-one (5), apigenin (6), steppogenin (7), glabranin (8); one cinnamic acid: chromenylacrylic acid (9); one isocoumarin: (R)-(-)-mellein (10); four triterpenoids: squalene (11), taraxeryl acetate (12), bauera-7,9(11)-dien-3b-yl acetate (13), oleana-11:13(18)-dien- 3b-yl acetate (14); six steroids: the mixture of b-sitosterol (15) and stigmasterol (16), the mixture of b-sitostenone (17) and stigmasta- 4,22-dien-3-one (18), the mixture of b-sitosterol-3-O-b-D-glucoside (19) and b-stigmasterol-3-O-b-D-glucoside (20). The structures of these compounds were determined by spectroscopic analysis.
Among these isolates, 2 and 3 are new compounds from the nature. These isolates were screened their cytotoxicities against HepG2 [human hepatoma: HBV Ag (-)], PLC/PRF/5 [human hepatoma: HBV Ag (+)], THP-1 (acute monocytic leukemia) and Raji (lymphoma) cancer cell lines in vitro. Compounds 4 and 6 exhibited cytotoxicity against HepG2, PLC/PRF/5 and Raji cancer cell lines.

並列關鍵字

Ficus formosana f. formosana

參考文獻

參考文獻

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