高效能液相層析法對塑膠玩具中鄰苯二甲酸酯類塑化劑之應用分析研究

The determination of phthalate esters plasticizers in plastic toys by high performance liquid chromatography

鐘惠慈(Huei-Cih Jhong)

指導教授 ： 柯黃盛

高雄醫學大學/藥學院/藥學研究所/碩士(2014年)

https://doi.org/10.6832/KMU.2014.00042

摘要

鄰苯二甲酸酯類(Phthalates esters, PAEs)常被當作塑化劑，普遍用於塑膠製品之中，且會隨著環境的不同，製造成不同軟硬程度的商品，也常添加運用於食品包裝、塗料、化妝品、容器、醫療器材、玩具之中。然而在高溫和高壓的環境之下，PAEs會隨著不同的途徑揮發到環境之中造成汙染，其中常見的PAEs像是Butyl benzyl phthalate (BBP)、Di-butyl phthalate (DBP)、Di(2-ethylhexyl) phthalate (DEHP)、Di-isononyl phthalate (DINP)、Di-n-octyl phthalate (DNOP)，而BBP、DBP、DEHP被歸類為內分泌干擾物質(環境賀爾蒙)，在動物方面有致癌毒性，對人體方面也會造成生殖方面之影響，因此本研究建立一個簡單及快速的高效能液相層析-紫外光偵測器(HPLC-UV)的分析方法，進行鄰苯二甲酸酯類塑化劑的分析檢測。
本實驗使用Phenomenex Kinetex C18(100 x 3.0 mm, 2.6 μm)作為分離層析管柱，並選用乙腈和水之混合溶液(90:10，v/v)，流速0.5 mL/min作為移動相進行沖提，以紫外光偵測器(UV detector)進行待測物之偵測，波長設定為202 nm。DEHP偵測範圍為1~200 μg/mL，偵測極限為0.5 μg/mL(S/N = 3，注入體積20 μL)，本研究可有效應用於檢測市售相關孩童玩具用品之塑化劑含量分析。除此之外，本實驗也對於五種不同種的塑化劑進行選擇性試驗分析條件探討，使用SGE protel Col Enduro C18(250 x 4.6 mm, 5 μm)之管柱在移動相為乙腈和水混合溶液(90:10，v/v) 比例之下，BBP、DBP、DEHP、DINP和DNOP能有效達到分離之效果。

關鍵字

塑膠玩具 ； 鄰苯二甲酸酯類 ； 高效能液相層析法 ； 紫外光偵測

並列摘要

Phthalates esters (PAEs) are widely used as plasticizers in plastic products, such as polyvinyl chloride (PVC), PVC products including vinyl flooring, baby toys, paints, cosmetics, food storage products, containers and medical devices, etc. However, the PAEs are only physically not chemically, bound to the polymer chain. If under high temperature and high pressure, plasticizers will release into the environment through volatilization. Some PAEs, such as butyl benzyl phthalate (BBP)、dibutyl phthalate (DBP) and di(2-ethylhexyl) phthalate (DEHP), have been studied on their metabolism and toxicity in mammals and environmental distribution. This study was based on the determination of PAEs by high performance liquid chromatography – UV detection (HPLC-UV) at 202 nm. The PAEs were separated on a Phenomenex Kinetex C18 (100 x 3.0 mm, 2.6 μm) column with the mobile phase consisting of acetonitrile: H2O (90:10, v/v) used in isocratic mode. Flow rate was 0.5 mL/min. The detection range of DEHP was about 1~200 μg/mL. The limit of detection was 0.5 μg/mL (S/N = 3, injection volume = 20 μL). Application of the method to the analysis of PAEs in the plastic toys and plastic products proved feasible.

並列關鍵字

Plastic toys ； Phthalates esters ； Di(2-ethylhexyl) phthalate ； High performance liquid chromatography- UV detection

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