利用雷射脫附游離質譜法進行合金及生物組織中金屬離子的檢測與成像分析

Metal Characterization of Alloys and Imaging Analysis of Tissues Using Laser Desorption Ionization Mass Spectrometry

謝中浩(Christopher Stephen Shiea)

指導教授 ： 黃友利

高雄醫學大學/健康科學院/醫學檢驗生物技術學系碩士班/碩士(2015年)

摘要

金屬、脂質及蛋白質是構成生物體的基本成份並具有重要的生理與生化功能。此外，金屬也廣泛地存在於食品及環境中並被應用於各類產業製程中。這些生物體內重要成份的濃度或分佈產生異常狀態的話，將可做為鑑別診斷疾病的可能病因並進而發展成為疾病的生物標記。質譜儀(mass spectrometer)具備優異與高靈敏度的分析特性，可用來偵測這些成份，但需要不同儀器分別進行無機成份(金屬離子) 及有機成份(生物分子)分析。因此，本研究利用有機質譜法進行無機及有機分析物的偵測及成像分析，以開發單一分析平台進行無機成份及有機成份分析為主要研究方向。本研究首先應用基質輔助雷射脫附游離質譜法(matrix-assisted laser desorption/ionization mass spectrometry, MALDI-MS)進行真實樣品中金屬離子的偵測並探究生物及臨床組織中的質譜成像分析。組織中金屬的成像分析結果與組織中脂質與蛋白質的組成之間的相關性分析將可凸顯本研究所開發方法應用在臨床鑑別與診斷的可能潛力。本研究第二部份為在無需樣品前處理下，利用大氣電噴灑雷射脫附游離質譜法(electrospray laser desorption ionization mass spectrometry, ELDI/MS)，配合螯合劑輔助金屬離子偵測，發展出固體樣品中金屬分析的新穎方法並成功地應用於含金屬成份固體樣品中各類金屬的偵測分析。

關鍵字

微量金屬 ； 螯合劑 ； 大氣質譜法 ； 電噴灑雷射脫附游離 ； 基質輔助雷射脫附游離 ； 成像質譜法

並列摘要

Metals are ubiquitous in food, industry, and the environment. Metals, lipids, and proteins are important biological and physiological components in organisms. Abnormal distributions of these analytes are disease factors and markers. Mass spectrometry (MS) can detect these analytes because of its high sensitivity, but different instrumentations are used to analyze inorganic metals and organic biomolecules. Using one analytical platform will make inorganic and organic MS analysis more simple and convenient. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) was used to detect metals in real samples and to image tissues. Chelating agents were used to bind and ionize metals in food, drug, and water samples. This new technique successfully uses one analytical platform to analyze inorganic and organic analytes, also enabling a holistic understanding of tissues for clinical diagnosis. As the analysis of intact metal solids has not been performed using ambient MS, a technique known as electrospray laser desorption ionization mass spectrometry (ELDI/MS) was used to characterize metal solids without pretreatment. Metals in solid aluminum, copper, nickel, and lead samples were detected, as well as those in coins.

並列關鍵字

Metal ions ； chelating agents ； ambient mass spectrometry ； electrospray laser desorption ionization ； matrix-assisted laser desorption/ionization ； imaging mass spectrometry

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