女貞子對苯甲醇丁酯誘發大鼠急性氧化壓力之保護作用評估

Protective effects of the fruit of Ligustrum lucidum on acute butylated hydroxytoluene-induced oxidative stress in rats

林卉梅(Hui-Mei Lin)

指導教授 ： 林俊清

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2006年)

摘要

女貞子是木犀科植物女貞（Ligustrum lucidium）之乾燥成熟果實，在傳統醫藥歸類於補益功能之常用生藥。本研究係探討女貞子乙醇萃取物對高劑量苯甲醇丁酯（Butylated hydroxytoluene，BHT）所誘發大鼠急性氧化壓力之保護作用評估。動物實驗模式將大鼠分為溶媒控制組（10 mL/kg 橄欖油）及誘發組（1000 mg/kg BHT），分別連續七天給予大鼠純水後，再分別管餵10 mL/kg 橄欖油及1000 mg/kg BHT來誘發大鼠急性氧化壓力；而藥物組則以三種不同劑量之女貞子（250、500及1000 mg/kg）來替代，再管餵1000 mg/kg之BHT。由結果顯示，與誘發組相比，女貞子乙醇萃取物能有效降低大鼠血清GPT、GOT、Alk-P、BUN、Creatinine（Cr）、LDH、Triglyceride（TG）與肺部灌流液LDH之含量，並能改善肝、肺組織脂質過氧化之程度（P < 0.05）。由組織病理切片觀察得知，女貞子乙醇萃取物可減輕大鼠肺部組織之損傷，此外，女貞子乙醇萃取物亦能有效提升大鼠肝、腎、肺臟中的抗氧化酵素之活性，如Catalase、SOD及GSH-Px等（P < 0.05），進而達到預防氧化壓力引起的器官損傷。因此，給予女貞子乙醇萃取物可藉其抗氧化壓力作用來預防BHT所引起的氧化傷害。

關鍵字

苯甲醇丁酯 ； 女貞子 ； 氧化壓力

並列摘要

Nüzhenzi (NZZ) is a dry fruit of Ligustrum lucidum Ait. (Oleaceae) that applied as tonic for kidney, liver and commonly prescribed in traditional Chinese medicine. The aim of this study was to investigate the effects of ethanol extract of fruit of L. lucidum (ELL) on butylated hydroxytoluene (BHT)-induced oxidative stress in rats. Animals were pre-treated with ELL (250, 500 and 1000 mg/kg/day, orally) for seven days, and then administrated with BHT (1000 mg/kg) by oral. Compared to the BHT (1000 mg/kg)-treated group, results showed that ELL could significantly reduce the levels of serum glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), alkaline phosphatase (Alk-P), blood urea nitrogen (BUN), creatinine (Cr), lactate dehydrogenase (LDH) and triglyceride (TG), as well as LDH in BALF. It also significantly decreased the level of lipid peroxidation in liver and lung. In addition, ELL also significantly enhanced the levels of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in liver, kidney and lung tissues in rats when compared with the BHT-induced group (P < 0.05). Finally, the histopathological evaluation of the tissues revealed that ELL reduced the incidence of lung lesions. According to the results, the protective effect of ELL against acute BHT-induced oxidative stress in rats could be through its antioxidative activities and the upregulation of antioxidative enzymes.

並列關鍵字

Butylated hydroxytoluene ； Ligustrum lucidum ； Oxidative stress

參考文獻

1.林俊清。應用生藥學（I）生藥與自由基。富山出版社。1995，p.1-9。

2.Cerutti P.A., Prooxidant states and tumor promotion, Science, 1985, 25, 227(4685), 375-81.

3.Lavrovsky Y., Chatterjee B., Clark R.A. and Roy A.K., Role of redox-regulated transcription factors in inflammation, aging and age-related diseases, Exp. Gerontol., 2000, 35, 521–32.

4.Shi L., Ma Y. and Cai Z., Quantitative Determination of Salidroside and Specnuezhenide in the Fruits of Ligustrum lucidum Ait. by High Performance Liquid Chromatography, Biomed. Chromatogr., 1998, 12, 27–30.

5.He Z.D., But P.P.H., Chan T.W., Dong H., Xu H.X., Lau C.P. and Sun H.D., Antioxidative Glucosides from the Fruits of Ligustrum lucidum, Chem. Pharm. Bull., 2001, 49(6), 780-4.