以蛋白質分析平台探討氬氣微電漿照射血液後之蛋白質變化

Using proteomic profiling analysis platform to identify blood proteins after argon micro plasma exposure

黃銘啟(Ming-Chi Huang)

指導教授 ： 田育彰

高雄醫學大學/健康科學院/醫學影像暨放射科學系碩士班/碩士(2015年)

摘要

當有傷口產生，傷口就會產生許多相關的機制來修復。除了附近的微血管通透性增加外，受損的血管也會產生一系列的反應，誘發凝血因子產生血栓與纖維蛋白。嚴重的出血，就會考慮使用電燒、雷射這兩種方式。對於糖尿病的患者而言，在肢體末端受傷後，由於血液的循環不良使傷口不易癒合，容易因反覆感染的情形而導致需要截肢。氬氣微電漿可以促使血液的凝結，也會促進血漿中的纖維蛋白產生。為了探討氬氣微電漿對於糖尿病患者的影響，本研究分別對紅血球及血漿進行照射。在紅血球的外觀以及過氧化數值的改變，可以發現糖尿病患者的紅血球比較不穩定；血漿中的蛋白質，在接受氬氣微電漿照射後，可以發現蛋白質可能出現的表現改變。

關鍵字

氬氣微電漿 ； 凝血 ； 糖尿病

並列摘要

When a wound was formed, it may induce many related mechanisms to heal the wound. Not only the blood capillary permeability increased, but also the damaged blood vessel may induce coagulation factors activation to produce thrombus and fibrins. To the severe bleeding, electrosurgical unit and laser may consider to stop bleeding. For the diabetic patients, when a wound was formed, it was hard to heal due to bad blood circulation. In that case, it may repeatedly cause infection. The argon micro plasma can promote blood coagulate and plasma fibrins produce. To explore how the argon micro plasma influences blood coagulation, the erythrocytes and plasma were irradiated by argon micro plasma in this study. In the morphology of erythrocytes and the concentrations of erythrocytes and plasma, diabetic blood was easily influenced after irradiation. The LC-MS/MS and the MALDI-MS were used to identify plasma proteins after argon micro plasma exposure. Between the different exposure times, different proteins were identified.

並列關鍵字

argon micro plasma ； coagulation ； diabetes mellitius

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