利用傅立葉轉換紅外線光譜儀(FTIR)進行黃芩萃取物及其活性成分之分析

Fourier Transforms infrared spectrometer (FTIR) using to carry on the Scutellaria extract and the active constituent analysis

楊婉瑜(Wan-Yu Yang)

指導教授 ： 蔡義弘

高雄醫學大學/藥學院/臨床藥學研究所/碩士(2008年)

https://doi.org/10.6832/KMU.2008.00087

摘要

黃芩為最被廣泛使用的傳統中草藥，其根具有解熱、抗發炎作用；活性成分為Baicalin Baicalein Wagonin，曾經使用HPLC 、TLC、 HSCCC、CE及MEKC對其活性成分做定性及定量的分析，本研究嘗試使用FTIR microspectrascopy做定量分析。
本研究先用不同溶媒萃取不同劑量的黃芩，以FTIR microspectrascopy 分析取得萃取液的紅外線光譜圖，研究吸收峰的高度及面積和黃芩萃取液劑量兩者間有無線性關係；研究顯示使用乙醇（85%）和乙酸乙酯（15%）混合溶媒的萃取液和紅外線光譜1687.31~1635.77 cm-1的吸收峰總面積最具線性關係，其R2 為0.9229。另把同一波峰總面積對HPLC分析所得的Baicalein含量做線性迴歸分析，得到R2為0.9166，證明具有線性關係，其迴歸方程式為：Y=9.0315X－7.3485。 接著將市售品之萃取液的紅外線波峰總面積帶入上述的迴歸方程式，得到活性成分含量， 與HPLC測得實際上萃取液的黃芩活性成分Baicalein含量做對照並計算準確度。結果為0.08g市售品Baicalein含量3.06~2.43mg/mL和HPLC分析出該萃取液的Baicalein含量5.04~1.86mg/mL比較所得的準確度為133~60％。本研究顯示使用FTIR作定量分析具有一定的準確度和線性關係。

關鍵字

傅立葉轉換紅外線光譜儀 ； 黃芩萃取物

並列摘要

Scutellaria baicalensis geogi , the Chinese herb which is widely used by people. Its root can be used to bring down a fever and anti-inflammation . the active compound of Scutellaria baicalensis geogi are baicalin 、baicalein and wagonin. Scholars have gained the quantitative and qualitative determination of these active components by various analytic methods (such as HPLC、HSCCC、 CE and MEKC ) but FTIR
In this research, FTIR is used to quantitate the extract of Scutellaria baicalensis geogi and try to find the linear relationship between absorption peak (peak height and peak area) and the dose of Scutellaria baicalensis geogi extract. The research shows obvious linear relationship between dose of Scutellaria baicalensis geogi and absorption peak area of the band (1687.31~1635.77 cm-1 ). It is 0.9229 to get R2.
Another analyze the same crest the whole area to HPLC resulting Baicalein content make regression analyze, it is 0.9166 to get R2, proves that has linear relations, it returns to equation preface Y=9.0315X－7.348
Then bring the total area of the infrared aborption peak of the extraction liquid of the commercial product into the return equation preface described above, get the active composition content, And then measure the active composition Baicalein content of the Scutellaria baicalensis geogi of extracting the liquid with HPLC in fact to make and contrast and calculate accuracy , The result is that Baicalein content of 0.08g commercial product (3.06~2.43mg/mL )analyses Baicalein content (5.04~1.86mg/mL )that should extract the liquid with HPLC and compares incomes Accuracy 133~60%.
This research reveals that uses FTIR to make a quantitative analysis and have certain accuracy and linear relation.

並列關鍵字

Fourier Transforms infrared spectrometer (FTIR) ； Scutellaria extract

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