木臘樹對肝癌細胞株毒殺效果、抗氧化、及抗四氯化碳誘發小鼠急性肝炎之保肝活性研究

The Cytotoxicity, Antioxidant, and the Hepatoprotective Effects on Carbon Tetrachloride-intoxication in Mice of Rhus succedanea L.

林宏隆(Hung-Lung Lin)

指導教授 ： 顏銘宏

高雄醫學大學/藥學院/藥學研究所碩士在職專班/碩士(2009年)

摘要

木臘樹 (Rhus succedanea) 為漆樹科 (Anacardiaceae) 漆樹屬 (Rhus) 的落葉喬木，別名：山漆、野漆，分布印度、華南至日本；產於台灣全島山麓，傳統典籍記載具有散瘀止血、解毒功能；主治急慢性肝炎、胃痛、跌打損傷等。本研究進行木臘樹嫩枝熱水及乙醇萃取物對急性肝炎保肝活性、抗氧化及肝癌細胞株毒殺效果之研究。
抗氧化體外試驗使用 α,α-diphenyl-β-picrylhydrazyl(DPPH) 清除自由基試驗評估抗氧化能力，結果木臘樹嫩枝之熱水及乙醇萃取物皆具有極強的清除自由基的活性，但以熱水萃取物表現最好；乙醇萃取物之有機分層 ( n-Hexane、Chlorform、n-Butanol、有機水層)中以n-Butanol 層抗氧化效果最好。
Huh 7肝癌細胞株毒殺效果實驗顯示木臘樹嫩枝乙醇萃取物及其有機溶媒劃分層（特別是n-Butanol層）之細胞毒殺效果顯著。
保肝體內動物模式研究，ICR雄性小白鼠口服投與木臘樹嫩枝熱水、乙醇萃取物（水飛薊素Silymarin為陽性對照組）三天，每天兩次，最後一次投與後禁食十二小時，給予四氯化碳腹腔注射，六小時後犧牲動物，心臟採血分離血清檢測 GOT、GPT、LDH之值，並將肝組織以 H&E染色作病理分析。體內保肝研究結果顯示木臘樹嫩枝熱水萃取組可以有效降低肝功能酵素GPT及細胞存活酵素指標LDH 之值，其效果比水飛薊組更顯著，但乙醇萃取組效果不顯著。肝均質液抗脂質過氧化結果，木臘樹嫩枝熱水萃取組可以有效降低MDA含量，顯示木臘樹嫩枝熱水萃取物具有抗氧化功能，但不影響肝臟穀胱???式]Glutathione）之值。光學顯微鏡鏡檢肝組織病理變化，顯示四氯化碳誘發組肝臟中央靜脈週邊組織有明顯細胞壞死、脂肪變性之病變，木臘樹嫩枝乙醇萃取物組改善效果不顯著，熱水萃取物組明顯改善肝組織病變情況，結果與肝功能檢測相互吻合。

關鍵字

木臘樹 ； 四氯化碳 ； 急性肝炎 ； 抗氧化 ； 肝癌細胞毒

並列摘要

Rhus succedanea L., also called Wax tree, Sumach, is a species of genus Rhus that grows in East Asia, in regions of China, Japan, and Taiwan. It is sometimes used in Chinese medicine for the treatment of stypsis, gastralgia, acute and chronic hepatitis. The aim of the present study was to assess the cytotoxicity, antioxidant and hepatoprotective effects of R. succedanea against carbon tetrachloride (CCl4) - induced acute hepatitis in vivo.
Antioxidant activities of the aqueous and ethanol extracts of R. succedanea branches, as well as n-hexane, chlorform, n-butanol, water fraction from the ethanol extract were examined by 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. In the DPPH system, the antioxidant activity of the aqueous extracts was superior to that of the ethanol extracts. Among the four organic fractions, the n-butanol fraction showed the highest scavenging activity.
In addition, we evaluated the cytotoxic effect of R. succedanea extracts on Huh 7 human hepatoma cell line. The results indicated that the ethanol extracts of R. succedanea branches, and n-butanol fraction from the ethanol extract exhibited anti-hepatoma activity.
Finally, we evaluated the hepatoprotective effects against CCl4- induced hepatotoxicity in mice. The R. succedanea aqueous extract at an oral dose of 500 mg/kg showed a significant hepatoprotective effect by reducing levels of glutamate pyruvate transminase (GPT) and lactic dehydrogenase (LDH) in serum, and malondialdehyde in liver homogenates. In addition, the same aqueous extract prevented the hepatotoxicity induced by CCl4 - induced intoxication as observed when assessing the liver histopathology. In conclusions, these data suggested that R. succedanea can prevent hepatic injuries from CCl4-induced hepatotoxicity in mice.

並列關鍵字

Rhus succedanea ； carbon tetrachloride ； acute hepatitis ； antioxidant, cytotoxicity

參考文獻

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