台灣漢族華人牙周炎和IL-17A-692基因多型性相關性

The Association between IL-17A-692 Gene Polymorphism and Periodontal Disease in a Taiwan Han Population

劉嘉芳(Chia-Fang Liu)

指導教授 ： 何坤炎

高雄醫學大學/口腔醫學院/牙醫學研究所/碩士(2012年)

https://doi.org/10.6832/KMU.2012.00084

摘要

研究目的：有一種新分類的CD4+ T細胞，被命名為Th17細胞。而Th17細胞分泌一種促發炎的的細胞激素，IL-17A。本研究主要討論是IL-17A-692的基因多型性在台灣漢民族對於牙周炎的影響。
研究方法：共270個樣本中，依臨床口內檢查分為三組：牙周健康組（HC,N=64），慢性牙周炎組（CP,N=122）、侵犯性牙周炎組(AgP,N=84)。並萃取樣本白血球中DNA，以Real Time Taqman probe PCR技術確認IL-17A-692 (rs8193036)基因型
結果： IL-17A-692基因型在總樣本與未抽菸樣本中，慢性牙周炎和侵犯性牙周病組與牙周健康組在統計學上沒有顯著的差異。但在抽菸者樣本中，於牙周病組與牙周健康組進行統計分析，基因型上有明顯差異（p=0.0158）。在調整年齡與性別變項後CC+CT與TT對於牙周病的影響並無顯著（0.096）。
結論：本研究探討IL-17A(rs8193036)單一核苷酸基因多型性和牙周炎之相關性，研究結果顯示抽菸樣本中牙周健康組與慢性牙周炎組IL-17A-692的基因型分佈有顯著的不同（p=0.0103）。
同樣在抽煙樣本中牙周健康組與牙周炎組IL-17A-692的基因型分佈也有顯著不同。因此我們也可以推論抽菸可能會影響IL-17A-692的基因型表現。

關鍵字

介白質-17A ； 基因多型性 ； 牙周炎

並列摘要

Objectives: A new subset of CD4+ T cells was discovered and has been named as Th-17 cells. Th-17 cells secret pro-inflammatory cytokine IL-17A. The purpose of this study was to investigate the association of single nucleotide polymorphisms of IL-17A-692 and the risk of developing periodontitis in Taiwan Han population.
Material and method: There were 270 subjects which were classified as health control group (HC, N=64), chronic periodontitis (CP, N=122), and aggressive periodontitis (AgP, N=84) .The SNP of IL-17A-692 (rs8193036) was determined by Real Time Tagman probe PCR.
Result: The genotype distribution in the smoking group showed significantly different between the HC and periodontitis (p = 0.0158) After the adjustments of variations such as sex, age, the prevalence of the CC+CT genotype compared with the TT genotypes was no longer significantly different (p=0.096) .
Conclusion: The result indicated the genotype distribution was significant different between HC and CP in smoking group（p=0.0103）, and the HC and periodontitis group was significant. We presume the smoking could interfere the behavior of genotype.

並列關鍵字

IL-17A ； Periodontitis ； SNP

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