大武新木薑子根部之化學成分及抗發炎活性之研究

Chemical Constituents and Anti-inflammatory Activity from the Root of Neolitsea daibuensis

黃書鈴(Su-Ling Wong)

指導教授 ： 陳益昇

高雄醫學大學/藥學院/天然藥物研究所碩士班/碩士(2010年)

摘要

大武新木薑子(Neolitsea daibuensis)為屬樟科之半落葉性小喬木，為台灣固有種。分佈在台灣南部海拔800至1,000公尺高的闊葉林中。在化學成分研究方面本植物過去僅有一個生物鹼S-(+)-reticuline由根材與根皮中獲得，其生物活性則尚未被研究過。
近來約有40種的台灣樟科植物測試過其抑制iNOS的抗發炎活性，其中本植物根部甲醇萃取物表現出極佳的抑制NO產生的效果，同時對RAW 264.7 cells並無細胞毒殺性。此外，本植物根部的化學成分尚未被研究過。
利用生物活性導向試驗，大武新木薑子根部乙酸乙酯層萃取物分離得三個新的生物鹼類化合物: daibucarbolines A-C (1-3), 三個新的sesquiterpene類: daibulactones A-B (4-5), daibuoxide (6), 以及二十個已知化合物，包括八個sesquiterpene類: linderalactone (37), pseudoneolinderane (41), linderadine (44), epicubebol methyl ether (7), eudesm-4(15)-ene-1β, 6α-diol (8), hiiranlactone C (9), (+)-8a-hydroxyelemol (10), isolinderalactone (66), sericealactone (67),兩個flavonoids: 7-O-methylnaringenin (11), prunetin (12),一個steroid: β-sitosterol (181), 以及八個alkanoids methyl linoleate (13), 2,3-dihydroxypropyl palmitate (14), methyl palmitate (15), methyl stearate (16), stearic acid (17), docosanoic acid (18), oleic acid (19)及α-tocopherylquinone (20)。這些化合物之構造均以光譜分析決定。
將以上化合物進行活性測試，daibucarboline A (1), hiiranlactone C (9), 7-O-methylnaringenin (11), prunetin (12)及isolinderalactone (66)展現出不錯的iNOS抑制活性。其IC50 數值分別為18.41 ± 0.47, 29.30 ± 0.92, 19.55 ± 0.89, 10.50 ± 0.33 以及0.30 ± 0.01 μM。

關鍵字

大武新木薑子 ； 根部 ； 抗發炎

並列摘要

Neolitsea daibuensis Kamikoti (Lauraceae) is a small semideciduous trees, endemic to Taiwan, confined to broad-leaved forests from 800 to 1,000 m in the southern part. Only one alkaloid, S-(+)-reticuline, was previously isolated from the root wood and root bark of this plant. The biological activity of this species has
never been conducted before.
Recently, approximately 40 species of Formosan Lauraceous plants have been screened for anti-inflammatory activity using an inducible nitric oxide synthase (iNOS) assay, and the methanolic extract of the root of this species has been shown with potent inhibition of NO production without any cytotoxicity on RAW 264.7 cells. Besides, the chemical constituents of its root have not extensively been studied yet.
Bioassay-guided fractionation of the ethyl acetate soluble layer of the root of this species led to the isolation of three new alkaloids: daibucarbolines A-C (1-3), three new sesquiterpenoids: daibulactones A-B (4-5), and daibuoxide (6), along with twenty known compounds: linderalactone (37), pseudoneolinderane (41), linderadine (44), epicubebol methyl ether (7), eudesm-4(15)-ene-1β, 6α-diol (8), hiiranlactone C (9), (+)-8a-hydroxyelemol (10), isolinderalactone (66), sericealactone (67),two flavonoids, 7-O-methylnaringenin (11) and prunetin (12), one steroid, β-sitosterol (181), and eight alkanoids, methyl linoleate (13), 2,3-dihydroxypropyl palmitate (14), methyl palmitate (15), methyl stearate (16), stearic acid (17), docosanoic acid (18), oleic acid (19) and α-tocopherylquinone (20). The structures of these compounds were determined by spectroscopic analysis.
Among the isolates, daibucarboline A (1), hiiranlactone C (9),7-O-methylnaringenin (11), prunetin (12) and isolinderalactone (66) showed iNOS inhibitory activity with IC50 values as 18.41 ± 0.47, 29.30 ± 0.92, 19.55 ± 0.89, 10.50 ± 0.33 and 0.30 ± 0.01 μM, respectively.

並列關鍵字

Neolitsea daibuensis ； root ； anti-inflammatory

參考文獻

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