高良薑(Alpinia officinarum Hance)為薑科(Zingiberaceae)多年生草本植物,高良薑的根莖部位又名良薑、高良薑、高良姜、風薑和海浪薑,主產於廣東、廣西及臺灣等地。根莖部位含有黃酮類及 Diarylheptanoids 類化合物。先前研究發現高良薑具有抗癌、抗病毒、抗菌、抗發炎、抗突變及抗氧化等多項生物活性。 本研究以乾燥高良薑地下莖進行甲醇萃取、分配萃取,取得乙酸乙酯層、正丁醇 層及水層三個部分。 再將各分層萃取液進行初步藥理試驗 ,使用癌細胞毒殺效果及抗 氧化效果較佳的乙酸乙酯層進行管柱層析 、 高效能液相層析及再結晶方式進行分離純 化。純化之化合物,經由核磁共振光譜儀以及文獻比對,共鑑定二十三個化合物,分 別為一個固醇類、三個黃酮類、一個單萜類、兩個倍半萜類、兩個芳香類和十四個 Diarylheptanoids 化合物。
Alpinia officinarum Hance. ( Zingiberaceae ) is a perennial herb that was widely cultivated in Guangdong, Guangxi, and Taiwan. A. officinarum is also called Liang zang, Gau liang zang, Fong zang, and Hai lang zang. Previous studies have demonstrated that A. officinarum rhizomes contain a lot of chemical substances of flavonoids and diarylheptanoids. with anti-cancer, anti-virus, anti-bacterial, anti-inflammatory, anti-mutagenic and anti-oxidant activities. In this study, the air-dried rhizomes of A. officinarum were extracted with methanol. The MeOH extract was partitioned by ethyl acetate and butanol with water, respectively, to give EtOAc, n-BuOH and water layers. The extract of each layer was subjected to screen biological acitrvities and the result showed that the EtOAc layer of A. officinarum rhizomes have significant anti-oxidant and cytotoxic activities. Furthermore, these fractions from the EtOAc layer were purified by repeated column chromatography, high performance liquid chromatography and recrystallization. To obtain 23 compounds including one steroid, three flavonoids, one IV monoterpenoid, two sesquiterpenoids, two aromatics and fourteen diarylheptanoids. Their structures were elucidated by spectroscopic analysis including UV, EI-MS and NMR techniques.