牙周再生膜的製備與評估

Manufacturing and evaluating of periodontal regeneration membrane

戴安裕(An-Yu Tai)

指導教授 ： 黃琮濱

共同指導教授 ： 劉百栓(Bai-Shuan Liu)

長榮大學/健康科學學院/生物科技學系(所)/碩士(2009年)

https://doi.org/10.6833/CJCU.2009.00057

摘要

本研究的目的為研發新的非吸收性及可吸收性牙周再生膜片。進行體外實驗，評估牙周再生膜片對於人類類骨母細胞MG-63的影響，進而選出最適當地牙周再生膜片。本研究使用鈦金屬(CP-Ti)作為非吸收性再生膜片，鈦金屬使用不同濃度的硫酸進行表面改質(AT)；新的可吸收性再生膜片則是使用幾丁聚醣與綠梔子素製備，綠梔子素交聯幾丁聚醣/三鈣磷酸鹽複合膜(GCT)。我們以FE-SEM以及XRD分析非吸收性再生膜片鈦金屬的表面改質特性，結果顯示硫酸濃度大於30%才有改質的效果並產生約3∼4 μm的表面粗糙度。細胞培養的結果顯示，AT膜表面粗糙度越大、GCT複合膜交聯濃度越高，細胞數量越少。MTT分析的結果顯示AT膜對於細胞並不具有毒性，而高濃度GCT複合膜具有細胞毒性。礦化染色以及基因表現的結果顯示AT40的表面粗糙度有助於骨母細胞的礦化作用。本研究結果顯示，酸蝕鈦膜與GCT複合膜在牙周再生的領域中有潛在的應用性。

關鍵字

牙周再生膜 ； 鈦金屬 ； 幾丁聚醣 ； 骨母細胞 ； 表面粗糙度

並列摘要

The aim of this study is to investigate a novel non-biodegradable periodontal regeneration membrane. Evaluate the influence of the membrane on human osteoblast-like cells, MG-63, in vitro. According to the results, try to find the most appropriate membrane. In this study the commercial pure titanium (CP-Ti) was used as non-biodegradable membrane. To modify titanium surface via sulfuric acid treating (AT). In this study chitosan and genipin was used to prepare a novel and biodegradable periodontal regeneration membrane, genipin cross-linked chitosan/ tricalcium phosphate composite barrier (GCT). Characterize the modified titanium surface by FESEM and XRD. The results indicate the concentration of sulfuric acid larger than 30%, there is significantly effective on the membrane surface. After cell culture test, we found cells proliferation were significantly slow on roughness surface and high concentration GCT membrane. The results of MTT test revealed that titanium membranes and the GCT membranes with low concentration genipin are no cytotoxin to MG-63 cells. Mineralization staining and gene expression results showed that the surface roughness of AT40 could promote osteoblast differentiation. The results of this study provided that acid-etched titanium substrate and GCT membrane have potential application in guided tissue regeneration research.

並列關鍵字

Guided Tissue Regeneration ； titanium ； chitosan ； osteoblast ； surface roughness

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