遠紅外線生物效應在神經導管的應用

APPLICATION OF FAR-INFRARED RAY BIOEFFECT ON NERVE GUIDE CONDUITS

卓宛樺(Wan-Hua Jhuo)

指導教授 ： 黃琮濱

共同指導教授 ： 劉百栓

長榮大學/健康科學學院/生物科技學系(所)/碩士(2016年)

摘要

組織工程在重建受損組織被認為是較佳方法之一，神經導管提供一個讓受傷細胞生長的空間與方向引導神經再生。幾丁聚醣（chitosan)是生物可降解材料，奈米碳管（CNTs）具有良好機械強度與導電性，此外，明膠（gelatin)也是生物可降解性材料，便宜容易取得與製備，綠梔子素（genipin）是低毒性交聯劑，遠紅外線具良好生物效應。本研究之主要目的乃先將奈米碳管經幾丁聚醣修飾，摻入明膠做為導管基材並以綠梔子素為交聯劑，製備成神經導管，並結合遠紅外線效應，利用表面觀察、細胞培養與分析來評估該導管材料的生物相容性。在實驗結果方面，成功製造引導神經再生導管，雖然細胞在材料生長情況，未達理想狀況，但在遠紅外線照射下細胞活性增加，同時也可以維持細胞在材料上的相容性。希望藉由材料整合遠紅外線和生醫材料的作用，以達到最佳化的加成效果。

關鍵字

幾丁聚醣 ； 奈米碳管 ； 明膠 ； 遠紅外線

並列摘要

Tissue engineering has been treated as a preferred method of treatment for reconstructing defective tissues in various biomedical engineering applications. Nerve cells at an injury site have to be guided in the appropriate direction to accelerate nerve regeneration. Chitosan was used as materials due to its good biocompatibility. Carbon nanotubes (CNTs) are an ideal reinforcing material due to their nanometer size, their extraordinary mechanical strength and high electrical conductivity. Gelatin is a biodegradable polymer with excellent biocompatibility, plasticity, and inexpensive and much easier to obtain. Compared with traditional crosslinking agents, genipin has less cytotoxicity and better biocompatibility. Incorporation of far-irradiation ray into a nerve conduit hope to improve peripheral nerve regeneration. The genipin cross-linked gelatin annexed with CNTs/chitosan as a nerve guide materials are produced. The far-infrared ray irradiation can increase the cell activity. We hope this composite has a good biocompatibility which is necessary for clinical usage. Through integration of far-irradiation ray and biomaterials, we hope to establish a new perspective of research.

並列關鍵字

chitosan ； nanotube ； gelatin ； far-infrared ray

參考文獻

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