應用於監測中大腦動脈阻塞之無線多通道近紅外光譜儀設計

在神經醫學當中，近紅外光譜術是一項全新的量測技術。他可以在非侵入式的量測之下得到含氧血紅素與缺氧血紅素的資訊，而這些資訊代表著我們腦中的血容量以及含氧的飽和度。因此近紅外光譜術非常有淺力用於非侵入式量測的方式，來觀察人腦因缺血缺氧引起的中風。但是在缺血缺氧中風方面，現今的研究仍缺乏有力的生理參數資訊。在此篇研究當中我們利用近紅外光譜術找出有意義的生理參數資訊。微透析裝置所量測到的L/P ratio在老鼠的中大腦動脈阻塞之下，也被用來與近紅外光譜術的含氧與缺氧血紅素做比較。在進行老鼠中大腦動脈阻塞的試驗之後，老鼠大腦會利用三苯基氯化染色來確認受損的情況。最後在缺血缺氧中風中，總和血紅素以及含氧與缺氧血紅素的差值也是重要的參數資訊。在實驗的結果當中，含氧與缺氧血紅素對於腦中風有明顯的生理意義，並且兩者的差值有更明顯的差別與生理意義。

關鍵字

中大腦動脈阻塞；含氧血紅素；微透析；缺氧血紅素；缺血缺氧中風；近紅外光譜術

並列摘要

Near-infrared spectroscopy is a novel measuring technology in neuroscience. It can be used to non-invasively measure the relative concentrations of oxy-hemoglobin (HbO2) and deoxy- hemoglobin (HbR) which can reflect the information related to cerebral blood volume and cerebral oxygen saturation. Therefore, it has the potential for noninvasive monitoring of cerebra ischemia. However, it still lacks reliable physiological information in cerebra ischemia. In this study, we attempt to find meaningful information related to cerebra ischemia from the relative concentrations of HbO2 and HbR measured by near-infrared spectroscopy. The value of L/P ratio measured by using microdylasis is also used to compare with the concentration variation of HbR and HbO2 under middle cerebral artery occlusion. The rat brain slices with triphenyltetrazolium chloride stain is made to confirm the infract region of the rat brain caused by middle cerebral artery occlusion. Finally, total haemoglobin concentration and concentration difference between HbO2 and HbR for cerebra ischemia are also investigated. The experimental results show the concentration variation of HbR and HbO2 certainly can reflect the state of cerebra ischemia, and the concentration difference between HbO2 and HbR can provide meaningful information for monitoring cerebra ischemia.

並列關鍵字

deoxy-hemoglobin ； ischemia stroke ； microdylasis ； middle cerebral occlusion ； near-infrared spectroscopy ； oxy-hemoglobin

參考文獻

[2]. N. R. Sims, H. Muyderman,” Mitochondria, oxidative metabolism and cell death in stroke,” Biochimica et Biophysica Acta, Vol. 1802, pp. 80–91,2010

[4]. M. E. Raichle,” The Pathophysiology of Brain Ischemia,” Neurological Progress, Vol. 13, pp. 2-10,1983

[5]. J. Karkela, M. Pasanen, S. Kaukinen, P. Morsky, A. Harmoinen," Evaluation of hypoxic brain injury with spinal fluid enzymes, lactate and pyruvate," Critical Care Medicine, Vol.20, pp.378-386, 1992

[6]. E. Ronne-Engstrom, H. Carlson, Y.S. Liu, U. Ungerstedt, L. Hillered,” Influence of perfusate glucose concentration on dialysate lactate, pyruvate, aspartate, and glutamate levels under basal and hypoxic conditions: a microdialysis study in rat brain,” Journal of Neurochemistry, Vol.65, pp.257-262, 1995

[7]. H. Ay, K. L. Furie, A. Singhal, W. S. Smith, A. G. Sorensen, W. J. Koroshetz,”An evidence-based causative classification system for acute ischemic stroke,” Annals of Neurology, Vol. 58, pp.688-697, 2005

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應用於監測中大腦動脈阻塞之無線多通道近紅外光譜儀設計

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